Milt production in the Japanese eel Anguilla japonica induced by repeated injections of human chorionic gonadotropin
Milt production in the Japanese eel Anguilla japonica induced by repeated injections of human chorionic gonadotropin
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DOI：
10.2331/fishsci.62.44
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发表时间：
1996-02-01
影响因子：
1.9
通讯作者：
Hirose, K
中科院分区：
文献类型：
--
作者：
Ohta, H;Kagawa, H;Hirose, K
在20 ℃海水中饲养的性未成熟雄性鳗鱼（平均体重253 g），每周（共14次）注射浓度为250或750 IU/鱼/周的人绒毛膜促性腺激素（HCG）。每次注射后2天，检查精液重量和精液质量的变化。250 IU和750 IU组的一些给药雄性动物在第5次注射后射精，大多数雄性动物在第6次注射后射精。在250 IU组中，随着注射重复次数的增加，精液重量逐渐增加。在第11 - 13次注射期间，其变得恒定（1-2 g），并在第14次注射后达到4.0 g。在第7 - 9次注射期间获得了精子活力的潜力，并且在用450 mM NaCl稀释后15秒时精子的活力百分比保持在约70%，直到第14次注射后。第7 ~ 9次注射期间的pH值（8.1-8.2）显著高于第10 ~ 11次注射期间。在整个实验过程中，两组雄性动物的血清17，20 β-二羟基-4-异戊烯-3-酮水平均保持较低（0.1-0.2 ng/ml），尽管它们显著高于生理盐水对照水平（0.05-0.08 ng/ml）。在750 IU组中，个体雄性之间的精液重量存在显著差异。在整个实验过程中，250 IU组每尾鱼的平均总鱼糜重量为9.4 g，750 IU组为6.1 g。结果表明，在10周内每周重复注射250 IU/鱼（约1 IU/gBW）的HCG对未成熟雄性鳗鱼的人工成熟是有效的。
Sexually immature male eels (253 g average body weight), reared in sea water at 20 degrees C, were injected weekly (14 times in total) with human chorionic gonadotropin (HCG) at a concentration of 250 or 750 IU/fish/week. Changes of the milt weight and milt quality were examined two days after each injection. Some of the treated males of both the 250 IU and 750 IU groups spermiated after the 5th injection, and most males spermiated after the 6th injection. In the 250 IU group, the milt weight gradually increased with increase in the repetitions of injection. It became constant (1-2 g) during the period of the 11th-13th injection, and reached 4.0 g after the 14th injection. Acquisition of potential for sperm motility was attained during the period of the 7th-9th injections and the percent motility of spermatozoa at 15 sec after dilution with 450 mM NaCl remained at approximately 70% until after the 14th injection. Milt pH (8.1-8.2) during the period of the 7th-9th injection was significantly higher than the 10th-11th injection period. Serum 17,20 beta-dihydroxy-4-pregnen-3-one levels in the males of both groups remained low (0.1-0.2 ng/ml) throughout the experiment, although they were significantly higher than the saline control levels (0.05-0.08 ng/ml). In the 750 IU group, there was a marked difference in milt weight between individual males. The mean total milt weight throughout the experiment per fish of the 250 IU group was 9.4 g, and that of the 750 IU group was 6.1 g. The results indicate that repeated weekly injections of HCG at 250 IU/fish (about 1 IU/gBW) over 10 weeks is efficient for the artificial maturation of immature male eels.