Inhibition of a starch-granule-bound protein leads to modified starch and repression of cold sweetening
Inhibition of a starch-granule-bound protein leads to modified starch and repression of cold sweetening
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DOI：
10.1038/nbt0598-473
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发表时间：
1998-05-01
影响因子：
46.9
通讯作者：
Kossmann, J
中科院分区：
文献类型：
--
作者：
Lorberth, R;Ritte, G;Kossmann, J
我们已经克隆了一个参与淀粉代谢的基因，该基因通过其产物与马铃薯淀粉颗粒结合的能力来鉴定。转基因马铃薯蛋白质水平的降低导致淀粉中磷酸盐含量的降低。当蛋白质在大肠杆菌中表达时获得互补结果，因为这导致糖原的磷酸盐含量增加。这种蛋白质可能负责将磷酸盐掺入淀粉样葡聚糖中，这是一个在生物化学水平上尚不清楚的过程。马铃薯淀粉中磷酸盐含量的降低对其降解性有一些次级影响，因为相应的植物在叶子中显示淀粉过量表型，并且在块茎中显示冷甜化减少。
We have cloned a gene involved in starch metabolism that was identified by the ability of its product to bind to potato starch granules. Reduction in the protein level of transgenic potatoes leads to a reduction in the phosphate content of the starch. The complementary result is obtained when the protein is expressed in Escherichia coli, as this leads to an increased phosphate content of the glycogen. It is possible that this protein is responsible for the incorporation of phosphate into starch-like glucans, a process that is not understood at the biochemical level. The reduced phosphate content in potato starch has some secondary effects on its degradability, as the respective plants show a starch excess phenotype in leaves and a reduction in cold-sweetening in tubers.