Multiple Evolutionarily Conserved Enhancers Control Expression of Eya1
Multiple Evolutionarily Conserved Enhancers Control Expression of Eya1
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DOI：
10.1002/dvdy.21716
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发表时间：
2008-11-01
影响因子：
2.5
通讯作者：
Kawakami, Kiyoshi
中科院分区：
文献类型：
--
作者：
Ishihara, Tadashi;Sato, Shigeru;Kawakami, Kiyoshi
Eya1 是果蝇中不存在的眼睛的同源物，对于脊椎动物的各种器官的形成至关重要。小鼠和雏鸡 Eya1 在早期发育中表现出动态表达模式。我们通过筛选进化保守序列鉴定了十个独立的 Eya1 增强子。它们在鸡胚 HH6-17 的亨森结、神经管、迁移神经嵴细胞、耳泡、嗅基板、颅神经节和体节中表现出增强子活性。增强子的增强子活性之和涵盖了小鸡和小鼠共有的Eya1内源表达域。在物种特异性表达域（如三叉神经节和大脑）中也观察到增强子活性。对其中一个增强子的突变研究表明，该增强子由正顺式调节元件和负顺式调节元件组成。因此，我们成功地鉴定了Eya1基因座周围的一组综合增强子，它们可能参与Eya1体内复杂表达模式的控制。发育动力学 237.3142-3156，2008。(C) 2008 Wiley-Liss, Inc.
Eya1 is a homolog of eyes absent in Drosophila, and essential for various organ formations in vertebrates. Mouse and chick Eya1 shows dynamic expression pattern in early development. We identified ten independent Eya1 enhancers by screening evolutionarily conserved sequences. They exhibited enhancer activities in Hensen's node, neural tube, migrating neural crest cells, otic vesicle, olfactory placode, cranial ganglia, and somites at HH6-17 of chick embryo. The sum of the enhancer activities of the enhancers covers the endogenous expression domains of Eya1 common to chick and mouse. Enhancer activities were also observed in species-specific expression domains such as trigeminal ganglia and brain. Mutational study of one of the enhancers revealed that the enhancer is composed of positive and negative cis-regulatory elements. Thus, we successfully identified a comprehensive group of enhancers around Eya1 locus, which are probably involved in the control of the complex expression pattern of Eya1 in vivo. Developmental Dynamics 237.3142-3156, 2008. (C) 2008 Wiley-Liss, Inc.