Protection against group A streptococcus by immunization with J8-diphtheria toxoid: Contribution of J8-and diphtheria toxoid-specific antibodies to protection
Protection against group A streptococcus by immunization with J8-diphtheria toxoid: Contribution of J8-and diphtheria toxoid-specific antibodies to protection
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DOI：
10.1086/374800
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发表时间：
2003-05-15
影响因子：
6.4
通讯作者：
Good, MF
中科院分区：
文献类型：
--
作者：
Batzloff, MR;Hayman, WA;Good, MF
A组链球菌（GAS）M蛋白的构象限制，最低限度保守的肽已被定义。它由来自非M蛋白螺旋形成序列内的C-重复区的12个氨基酸组成，被称为“J8”。“在这里，我们研究了J8-白喉类毒素（DT）结合物与人类相容性佐剂SBAS 2和明矾佐剂的免疫原性，并证明它能够诱导调理素抗体，并可以保护远交小鼠免受毒性攻击。在一系列实验中，保护作用与J8特异性抗体的滴度相关，而与J8特异性T细胞的诱导无关。然而，DT-特异性抗体（以及J8-特异性抗体）显示出染色固定GAS的表面，并能够调理活的生物体。DT可能是J8和其他GAS肽用于GAS疫苗的理想载体蛋白。
A conformationally constrained, minimally conserved peptide from the M protein of group A streptococcus ( GAS) has been defined. It consists of 12 amino acids from the C-repeat region within a non - M protein helix-forming sequence and is referred to as "J8." Here, we investigate the immunogenicity of a J8-diphtheria toxoid (DT) conjugate adjuvanted with the human-compatible adjuvants, SBAS2 and alum, and demonstrate that it is capable of inducing opsonic antibodies and can protect outbred mice from virulent challenge. In a range of experiments, protection correlated with the titer of J8-specific antibodies and not with the induction of J8-specific T cells. However, DT-specific antibodies ( as well as J8-specific antibodies) were shown to stain the surface of fixed GAS and to be capable of opsonizing live organisms. DT may be an ideal carrier protein for J8 and other GAS peptides for GAS vaccines.