Engineering of antibodies
Engineering of antibodies
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抗体工程
DOI：
10.1002/bies.950080207
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发表时间：
1988
期刊：
影响因子：
4
通讯作者：
L. Riechmann
中科院分区：
文献类型：
--
作者：
M. Verhoeyen;L. Riechmann
人单克隆抗体极难通过杂交瘤技术获得。作为替代方案，“类人”抗体已通过重组DNA技术产生。第一个这样的工程抗体由嵌合蛋白组成，其中鼠可变区与人恒定区连接。最近，通过将鼠抗体的结合位点移植到人抗体中，“人”抗体已经被“重塑”。更多的抗体已经被分解成结构域组（Fab，Fc），例如，Fab已经与酶连接，导致抗体-酶嵌合体。除了其潜在的临床应用，工程抗体将证明在可变区和恒定区的结构/功能关系的研究中是无价的。
Human monoclonal antibodies are extremely difficult to obtain by hybridoma technology. As an alternative, ‘human‐like’ antibodies have been produced by recombinant DNA technology. The first such engineered antibodies consisted of chimaeric proteins, in which murine variable regions were linked to human constant regions. More recently ‘human’ antibodies have been ‘reshaped’ by transplanting the binding site of a murine antibody into a human antibody. Further‐more antibodies have been dissected into groups of domains (Fab's, Fc's) and for example, Fab's have been joined to enzymes resulting in antibody‐enzyme chimaeras. In addition to their potential clinical applications, engineered antibodies will prove invaluable in studies of structure/function relationships of variable and constant regions.
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DOI：
10.1073/pnas.80.3.825
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
OI, VT;MORRISON, SL;BERG, P
通讯作者：
BERG, P
DOI：
10.1073/pnas.81.21.6851
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MORRISON, SL;JOHNSON, MJ;OI, VT
通讯作者：
OI, VT
影响因子：
56.9
作者：
MCCLARIN, JA;FREDERICK, CA;ROSENBERG, JM
通讯作者：
ROSENBERG, JM
人 IgE ε 链的生物活性片段在大肠杆菌中的表达。
DOI：
10.1073/pnas.81.17.5369
发表时间：
1984
影响因子：
11.1
作者：
Liu,FT;Albrandt,KA;Bry,CG;Ishizaka,T
通讯作者：
Ishizaka,T