Acceleration of membrane recycling by axotomy of cultured Aplysia neurons
Acceleration of membrane recycling by axotomy of cultured Aplysia neurons
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DOI：
10.1016/s0896-6273(00)80083-5
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发表时间：
1996-03-01
期刊：
影响因子：
16.2
通讯作者：
Spira, ME
中科院分区：
文献类型：
--
作者：
Ashery, U;Penner, R;Spira, ME
从静止的轴突快速转变为动态的生长锥体，需要膜的募集，并将膜策略性地插入神经膜。支持术后早期快速生长的膜的来源尚不清楚。利用膜电容测量，我们研究了培养的海兔神经元轴突切断后膜动力学的定量方面。轴突切断同时激活两个过程：膜回收和胞吐。出乎意料的是，在大多数实验中，膜提取是占主导地位的过程。因此，当生长锥体大力伸展时，神经元的总表面积会减少。我们认为，新形成的生长锥切断后的最初快速延伸阶段是由从神经膜快速恢复的细胞内膜池支持的。
The rapid transition of a stationary axon into a motile growth cone requires the recruitment of membrane and its strategic insertion into the neurolemma. The source of membrane to support the initial rapid growth postaxotomy is not known. Using membrane capacitance measurements, we examined quantitative aspects of membrane dynamics following axotomy of cultured Aplysia neurons. Axotomy activates two processes in parallel: membrane retrieval and exocytosis. Unexpectedly, membrane retrieval is the dominant process in the majority of the experiments. Thus, while a growth cone is vigorously extending, the total neuronal surface area decreases. We suggest that the initial rapid extension phase of the newly formed growth cone postaxotomy is supported by a pool of intracellular membrane that is rapidly retrieved from the neurolemma.