Induction of metamorphosis decreases nitric oxide synthase gene expression in larvae of the marine mollusc Ilyanassa obsoleta (Say)
Induction of metamorphosis decreases nitric oxide synthase gene expression in larvae of the marine mollusc Ilyanassa obsoleta (Say)
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DOI：
10.2307/4134543
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发表时间：
2006-12-01
影响因子：
1.6
通讯作者：
Leise, Esther M.
中科院分区：
文献类型：
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作者：
Hens, Mark D.;Fowler, Kenneth A.;Leise, Esther M.
许多海洋生物在其生命的早期是作为幼虫悬浮在水柱中，然后蜕变为底栖生殖成虫。在幼虫能够对适当的刺激作出反应之前，以及在遇到适合幼鱼的栖息地之后，变态才会发生。气体神经递质一氧化氮被认为是重要的，在变态的调节，通过保持生物体在幼虫状态。我们研究了神经元型一氧化氮合酶（nNOS）基因在海洋泥螺Ilyanassa obsoleta的幼虫和变态个体中的表达。我们的研究结果表明，nNOS在整个幼虫发育过程中以恒定的水平表达。与此相反，nNOS的表达显着下降，在第一个24小时的变态。我们的观察结果支持以前的研究结果表明，一氧化氮是存在于幼虫虽然能力。在变态过程中发生的nNOS基因表达的减少与先前描述的nNOS活性的减少相对应。
Many marine organisms spend the early part of their lives as larvae suspended in the water column before metamorphosing into benthic reproductive adults. Metamorphosis does not occur until a larva has become competent to respond to appropriate stimuli and after a suitable habitat for the young juvenile has been encountered. The gaseous neurotransmitter nitric oxide is thought to be important in the regulation of metamorphosis by holding the organism in the larval state. We have investigated expression of the neuronal nitric oxide synthase (nNOS) gene in larval and metamorphosing individuals of the marine mud snail Ilyanassa obsoleta. Our results indicate that nNOS is expressed at constant levels throughout larval development. In contrast, expression of nNOS decreases markedly during the first 24 h of metamorphosis. Our observations support previous findings that demonstrate that nitric oxide is present in larvae though competence. The decrease in nNOS gene expression that occurs during metamorphosis corresponds with a previously described reduction in nNOS activity.