Isolation of a functional vesicular intermediate that mediates ER to Golgi transport in yeast.
Isolation of a functional vesicular intermediate that mediates ER to Golgi transport in yeast.
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DOI：
10.1083/jcb.111.1.45
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发表时间：
1990-07
影响因子：
7.8
通讯作者：
Ferro-Novick, S
中科院分区：
文献类型：
--
作者：
Groesch, M E;Ruohola, H;Bacon, R;Rossi, G;Ferro-Novick, S
我们已经使用了一种体外试验，重建运输从内质网到高尔基体复合体在酵母中，以确定一个功能性囊泡中间转运到高尔基体。透性化酵母细胞在该测定中用作供体，释放生物化学上不同于供体ER组分的均一囊泡群体。分离的囊泡，含有后ER/前高尔基体形式的标志物蛋白前α因子，能够结合并融合外源添加的高尔基体膜。分离融合感受态囊泡的能力提供了直接证据，ER到高尔基体膜的转运是由离散的囊泡载体群体介导的。
We have used an in vitro assay that reconstitutes transport from the ER to the Golgi complex in yeast to identify a functional vesicular intermediate in transit to the Golgi apparatus. Permeabilized yeast cells, which serve as the donor in this assay, release a homogeneous population of vesicles that are biochemically distinct from the donor ER fraction. The isolated vesicles, containing a post-ER/pre-Golgi form of the marker protein pro-alpha-factor, were able to bind to and fuse with exogenously added Golgi membranes. The ability to isolate fusion competent vesicles provides direct evidence that ER to Golgi membrane transport is mediated by a discrete population of vesicular carriers.