FINITE PROLIFERATIVE LIFE-SPAN IN-VITRO OF A HUMAN BREAST-CANCER CELL STRAIN ISOLATED FROM A METASTATIC LYMPH-NODE
FINITE PROLIFERATIVE LIFE-SPAN IN-VITRO OF A HUMAN BREAST-CANCER CELL STRAIN ISOLATED FROM A METASTATIC LYMPH-NODE
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DOI：
10.1007/bf00666588
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发表时间：
1993-01-01
影响因子：
3.8
通讯作者：
ETHIER, SP
中科院分区：
文献类型：
--
作者：
MAHACEK, ML;BEER, DG;ETHIER, SP
我们最近描述了允许从某些患者样本的活检标本中增殖转移性人乳腺癌细胞的培养条件。这些条件导致了命名为SUM-44 PE的永生化细胞株的开发。这些相同的培养条件用于从单独的乳腺癌患者的转移性淋巴结分离人乳腺癌细胞株。将从该样本中分离的SUM-16 LN人乳腺癌细胞在无血清培养基或补充有胰岛素和氢化可的松的含血清培养基中培养。与在培养物中连续增殖超过两年的SUM-44 PE细胞不同，SUM-16 LN细胞在培养物中增殖约200天，并在经历细胞衰老之前经历15至20次群体倍增。该菌株的细胞增殖不超过第8代。SUM-16 LN细胞角蛋白-19呈阳性，核型为非整倍体。这些细胞过表达p53蛋白，并具有扩增的表皮生长因子（EGF）受体基因，导致酪氨酸磷酸化EGF受体蛋白的高水平表达。尽管这些细胞中存在高水平的酪氨酸磷酸化EGF受体，但它们在无血清、无EGF培养基中增殖，并且不分泌可检测水平的EGF样促有丝分裂生长因子。此外，这些细胞的生长受到所有浓度的外源性EGF和中和EGF受体抗体Mab 425的有效抑制。这些结果表明，高水平的酪氨酸磷酸化的EGF受体存在于这些细胞中的受体过表达的直接结果，而不是一个模拟配体的存在的结果。因此，SUM-16 LN代表表现出晚期人乳腺癌细胞的遗传和细胞特征的人乳腺癌细胞株。然而，这些细胞在培养中表现出有限的增殖寿命，表明细胞永生化不是所有人乳腺癌细胞表达的表型。
We recently described culture conditions that allow proliferation of metastatic human breast cancer cells from biopsy specimens of certain patient samples. These conditions resulted in the development of an immortalized cell strain designated SUM-44PE. These same culture conditions were used to isolate a human breast cancer cell strain from a metastatic lymph node of a separate breast cancer patient. The SUM-16LN human breast cancer cells isolated from this specimen were cultured either in serum-free medium or serum-containing medium supplemented with insulin and hydrocortisone. Unlike the SUM-44PE cells that have proliferated in culture continuously for over two years, SUM-16LN cells proliferated in culture for approximately 200 days and underwent 15 to 20 population doublings before undergoing cell senescence. No cells of this strain proliferated beyond passage 8. SUM-16LN cells were keratin-19 positive and had an aneuploid karyotype. These cells overexpressed p53 protein and had an amplified epidermal growth factor (EGF) receptor gene that resulted in high level expression of tyrosine phosphorylated EGF receptor protein. Despite the presence of high levels of tyrosine phosphorylated EGF receptor in these cells, they proliferated in serum-free, EGF-free medium and did not secrete detectable levels of EGF-like mitogenic growth factor. In addition, these cells were potently growth inhibited by all concentrations of exogenous EGF tested and by the neutralizing EGF receptor antibody Mab 425. These results suggest that the high level of tyrosine phosphorylated EGF receptor present in these cells is the direct result of receptor overexpression and not the result of the presence of a simulatory ligand. Thus, SUM-16LN represents a human breast cancer cell strain that exhibited genetic and cellular characteristics of advanced human breast cancer cells. Nevertheless, these cells exhibited a finite proliferative lifespan in culture, suggesting that cellular immortalization is not a phenotype expressed by all human breast cancer cells.