Estramustine-Induced Suicidal Erythrocyte Death
Estramustine-Induced Suicidal Erythrocyte Death
复制标题
DOI：
10.1159/000356580
复制
发表时间：
2013-01-01
影响因子：
--
通讯作者：
Lang, Florian
中科院分区：
文献类型：
--
作者：
Bissinger, Rosi;Modicano, Paola;Lang, Florian
背景：雌二醇-17 β-磷酸雌莫司汀的氮芥衍生物用于治疗前列腺癌。雌莫司汀可能引发癌细胞的自杀性死亡。雌莫司汀的副作用包括贫血。至少在理论上，雌莫司汀可能会通过刺激红细胞自杀性死亡而导致贫血。红细胞凋亡的特征包括细胞皱缩、胞质Ca 2+活性（[Ca 2 +]）增加、神经酰胺形成和磷脂酰丝氨酸移位至细胞膜的外小叶，并在红细胞表面暴露磷脂酰丝氨酸。胞浆Ca ~（2+）活性（[Ca ~（2+）]（i））的增加可刺激胞浆内Ca ~（2+）的分泌。本研究探讨雌莫司汀是否会引发眼睑下垂。研究方法：[Ca 2 +]（i）根据Fluo 3荧光、前向散射的细胞体积、膜联蛋白V结合的磷脂酰丝氨酸暴露和血红蛋白释放的溶血进行估计。结果：雌莫司汀24 h暴露（
Background: The nitrogen mustard derivative of estradiol-17 beta-phosphate estramustine is used for the treatment of prostate cancer. Estramustine may trigger suicidal death of cancer cells. Side effects of estramustine include anemia. At least in theory, estramustine could cause anemia by stimulation of eryptosis, the suicidal death of erythrocytes. Hallmarks of eryptosis include cell shrinkage, increased cytosolic Ca2+ activity ([Ca2+]), ceramide formation and phosphatidylserine translocation to the outer leaflet of the cell membrane with phosphatidylserine exposure at the erythrocyte surface. Eryptosis is stimulated by increase of cytosolic Ca2+ activity ([Ca2+](i)). The present study explored whether estramustine triggers eryptosis. Methods: [Ca2+](i) was estimated from Fluo3 fluorescence, cell volume from forward scatter, phosphatidylserine exposure from annexin V binding, and hemolysis from hemoglobin release. Results: A 24 h exposure to estramustine (