Sugar-responsive gene expression and the agr system are required for colony spreading in Staphylococcus aureus
Sugar-responsive gene expression and the agr system are required for colony spreading in Staphylococcus aureus
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DOI：
10.1016/j.micpath.2011.04.003
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发表时间：
2011-09-01
影响因子：
3.8
通讯作者：
Sekimizu, Kazuhisa
中科院分区：
文献类型：
--
作者：
Ueda, Tomofumi;Kaito, Chikara;Sekimizu, Kazuhisa
金黄色葡萄球菌在软琼脂表面扩散，这种扩散被称为“菌落扩散”。在这里，我们报告了在软琼脂平板中添加葡萄糖促进了金黄色葡萄球菌中的菌落扩散。参与分解代谢抑制的CCPA和hprK的破坏降低了葡萄糖促进的菌落扩散能力。AGR基因是一种毒力调节元件，其表达被葡萄糖以依赖于CCPA的方式激活，该基因的缺失取消了葡萄糖促进的菌落扩散。破坏ClpP和arlRs有助于agr的表达，也减少了葡萄糖促进的菌落扩散。这些发现表明，金黄色葡萄球菌的菌落传播需要AGR的表达，而AGR的表达受环境碳源的正向调节，在金黄色葡萄球菌的感染过程中，毒力基因的表达和AGR诱导的菌落扩散同时被激活。(C)2011爱思唯尔有限公司。保留所有权利。
Staphylococcus aureus spreads on soft agar surfaces, which is called "colony spreading". Here, we report that the colony spreading in S. aureus was promoted by the addition of glucose to soft agar plates. Disruption of ccpA and hprK, which are involved in catabolite repression, decreased the colony spreading ability promoted by glucose. Deletion of the agr locus, a virulence regulatory element whose expression is activated by glucose in a ccpA-dependent manner, abolished the colony spreading promoted by glucose. Disruption of clpP and arlRS, which contributes to agr expression, also decreased glucose-promoted colony spreading. These findings suggest that S. aureus colony spreading requires the expression of agr, which is positively regulated by environmental carbon sources, and that virulence gene expression and colony spreading induced by agr are simultaneously activated in the S. aureus infectious process. (C) 2011 Elsevier Ltd. All rights reserved.