Inhibition of Quorum Sensing Mediated Virulence Factors Production in Urinary Pathogen Serratia marcescens PS1 by Marine Sponges
Inhibition of Quorum Sensing Mediated Virulence Factors Production in Urinary Pathogen Serratia marcescens PS1 by Marine Sponges
复制标题
DOI：
10.1007/s12088-012-0272-0
复制
发表时间：
2012-06-01
影响因子：
3
通讯作者：
Ravi, Arumugam Veera
中科院分区：
文献类型：
--
作者：
Annapoorani, Angusamy;Jabbar, Abdul Karim Kamil Abdul;Ravi, Arumugam Veera
这项研究的重点是寻找一种使用抗生素对抗细菌感染的替代策略。针对紫色色杆菌 (ATCC 12472) 中酰基高丝氨酸内酯 (AHL) 介导的紫色素产生、CV026 和临床分离株粘质沙雷氏菌 PS1 中的毒力基因表达，评估了从印度帕克湾采集的海绵的群体感应抑制 (QSI) 活性。在测试的 29 块海绵中，Aphrocallistes bocagei (TS 8)、Haliclona (Gellius) megastoma (TS 25) 和 Clathria atrasanguinea (TS 27) 的甲醇提取物抑制 C. violaceum (ATCC 12472) 和 CV026 中 AHL 介导的紫罗兰素产生。此外，这些海绵提取物抑制了粘质沙门氏菌 PS1 中 AHL 依赖性灵菌红素色素、蛋白酶等毒力酶、溶血素的产生和生物膜的形成。然而，这些海绵提取物并不能抑制细菌生长，这表明这些提取物的 QSI 活性与对细菌的静态或杀灭作用无关。根据所获得的结果，预计海洋海绵可以为预防群体感应（QS）介导的细菌感染铺平道路。
The focal intent of this study was to find out an alternative strategy for the antibiotic usage against bacterial infections. The quorum sensing inhibitory (QSI) activity of marine sponges collected from Palk Bay, India was evaluated against acyl homoserine lactone (AHL) mediated violacein production in Chromobacterium violaceum (ATCC 12472), CV026 and virulence gene expressions in clinical isolate Serratia marcescens PS1. Out of 29 marine sponges tested, the methanol extracts of Aphrocallistes bocagei (TS 8), Haliclona (Gellius) megastoma (TS 25) and Clathria atrasanguinea (TS 27) inhibited the AHL mediated violacein production in C. violaceum (ATCC 12472) and CV026. Further, these sponge extracts inhibited the AHL dependent prodigiosin pigment, virulence enzymes such as protease, hemolysin production and biofilm formation in S. marcescens PS1. However, these sponge extracts were not inhibitory to bacterial growth, which reveals the fact that the QSI activity of these extracts was not related to static or killing effects on bacteria. Based on the obtained results, it is envisaged that the marine sponges could pave the way to prevent quorum sensing (QS) mediated bacterial infections.