The ion channel ppk301 controls freshwater egg-laying in the mosquito Aedes aegypti
The ion channel ppk301 controls freshwater egg-laying in the mosquito Aedes aegypti
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DOI：
10.7554/elife.43963
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发表时间：
2019-05-21
期刊：
影响因子：
7.7
通讯作者：
Vosshall, Leslie B.
中科院分区：
文献类型：
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作者：
Matthews, Benjamin J.;Younger, Meg A.;Vosshall, Leslie B.
雌性埃及伊蚊是虫媒病毒病原体的致命载体，并在与人类居住相关的淡水容器中繁殖。由于高盐度对后代是致命的，因此正确评估水的纯度是一项至关重要的育儿决定。我们发现 DEG/ENaC 通道 ppk301 和表达 ppk301 的感觉神经元控制着伊蚊的产卵起始和选择。埃及伊蚊。使用钙成像，我们发现表达 ppk301 的细胞对水表现出 ppk301 依赖性反应，但出乎意料的是，也以不依赖于 ppk301 的方式对盐做出反应。这表明 ppk301 在低盐浓度下对产卵有指导意义，但 ppk301 独立途径负责在高盐浓度下抑制产卵。水是昆虫生存的关键资源，了解蚊子如何与水相互作用以控制不同的行为是研究化学感应系统进化的机会。
Female Aedes aegypti mosquitoes are deadly vectors of arboviral pathogens and breed in containers of freshwater associated with human habitation. Because high salinity is lethal to offspring, correctly evaluating water purity is a crucial parenting decision. We found that the DEG/ENaC channel ppk301 and sensory neurons expressing ppk301 control egg-laying initiation and choice in Ae. aegypti. Using calcium imaging, we found that ppk301-expressing cells show ppk301-dependent responses to water but, unexpectedly, also respond to salt in a ppk301-independent fashion. This suggests that ppk301 is instructive for egg-laying at low-salt concentrations, but that a ppk301-independent pathway is responsible for inhibiting egg-laying at high-salt concentrations. Water is a key resource for insect survival and understanding how mosquitoes interact with water to control different behaviors is an opportunity to study the evolution of chemosensory systems.