MICROCALORIMETRIC MEASUREMENTS OF HEAT-PRODUCTION IN BROWN ADIPOCYTES FROM CONTROL AND CAFETERIA-FED RATS
MICROCALORIMETRIC MEASUREMENTS OF HEAT-PRODUCTION IN BROWN ADIPOCYTES FROM CONTROL AND CAFETERIA-FED RATS
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DOI：
10.1042/bj2350337
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发表时间：
1986-04-15
影响因子：
4.1
通讯作者：
NEVILLE, SD
中科院分区：
文献类型：
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作者：
CLARK, DG;BRINKMAN, M;NEVILLE, SD
顺序添加葡萄糖，去甲肾上腺素，普萘洛尔和油酸的O2消耗率和产热率的控制和自助餐喂养的大鼠分离的肩胛间棕色脂肪细胞的影响进行了比较。虽然化学试剂在氧化代谢方面产生了非常相似的变化，但当以每克干重表示时，细胞接近。65%，低于从对照大鼠获得的细胞。然而，当相同的结果表示每109多房棕色脂肪细胞，而不是重量分析，O2消耗和产热率是相等的。对这些数据的进一步解释是复杂的，因为来自于饲喂过燕麦的大鼠的多房棕色脂肪细胞的平均体积是来自于对照动物的多房细胞的2.5倍。
The effects of the sequential addition of glucose, noradrenaline, propranolol and oleic acid on the rates of O2 consumption and heat production by isolated interscapular brown adipocytes from control and cafeteria fed rats were compared. Although the chemical agents produced very similar changes in oxidative metabolism, the actual rates of O2 uptake and heat output in adipocytes from the cafeteria-fed rats, when expressed per g dry wt. of cells were approx. 65% less than those obtained with cells from the control rats. However, when the same results were expressed per 109 multiloccular brown adipocytes, rather than gravimetrically, rates of O2 consumption and heat production were equivalent. Further interpretation of these data is complicated, because the average volume of multiloccular brown adipocytes from cafeteria-fed rats was 2.5 times that for multiloccular cells from control animals.