Ectopic Expression of the Tomato Mi-1 Gene Confers Resistance to Root Knot Nematodes in Lettuce (Lactuca sativa)
Ectopic Expression of the Tomato Mi-1 Gene Confers Resistance to Root Knot Nematodes in Lettuce (Lactuca sativa)
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DOI：
10.1007/s11105-009-0143-y
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发表时间：
2010-06
影响因子：
2.1
通讯作者：
Li-Ying Zhang;Yuyang Zhang;Rugang Chen;Junhong Zhang;Taotao Wang;Hanxia Li;Z. Ye
中科院分区：
文献类型：
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作者：
Li-Ying Zhang;Yuyang Zhang;Rugang Chen;Junhong Zhang;Taotao Wang;Hanxia Li;Z. Ye
本研究对根结线虫（Meloidogyne spp.）本研究从番茄中克隆了抗病基因Mi-1，并将其转化生菜，研究其在异源系统中的功能。利用农杆菌介导法获得了含有Mi-1基因的转基因莴苣品系。转Mi-1基因植株中Mi-1基因的异位表达，提高了对根结线虫的抗性。
The full genomic region of the root knot nematode (Meloidogyne spp.) resistance gene Mi-1 was cloned from tomato and transformed into lettuce to investigate its function in a heterologous system. Transgenic lettuce lines containing the Mi-1 gene were developed using Agrobacterium-mediated transformation. Ectopic expression of the Mi-1 gene was observed in transgenic lines, and resistance to root knot nematode was improved.