Alterations in plasma biochemical composition in NO deficiency induced by L‐NAME in mice analysed by Fourier Transform Infrared Spectroscopy
Alterations in plasma biochemical composition in NO deficiency induced by L‐NAME in mice analysed by Fourier Transform Infrared Spectroscopy
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DOI：
10.1002/jbio.201600141
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发表时间：
2016-07
影响因子：
2.8
通讯作者：
Emilia Staniszewska‐Slezak;L. Mateuszuk;S. Chłopicki;M. Barańska;K. Malek
中科院分区：
文献类型：
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作者：
Emilia Staniszewska‐Slezak;L. Mateuszuk;S. Chłopicki;M. Barańska;K. Malek
采用NG -硝基- L -精氨酸甲酯（L - NAME）长期治疗引起的小鼠一氧化氮缺乏模型，对血浆进行红外光谱（FTIR）分析。L‐NAME导致外周阻力增加和全身性高血压。采用主成分分析（PCA）和线性判别分析（LDA）对光谱响应进行分类。PCA可以区分各组动物，表明FTIR光谱对一氧化氮缺乏症的发展敏感，这与表明高血压的血压值相反。在全球范围内，在L - NAME治疗的第二周和第三周观察到最明显的光谱变化，这表明血浆的红外特征可以作为疾病早期和晚期的指标。PLS‐DA方法提供了bb0 95%的分类准确率。L‐NAME处理的光谱特征主要与蛋白质水平升高、酪氨酸含量降低和脂质/磷脂浓度变化有关。在我们的工作中，我们讨论了这些变化，在L - NAME处理的每个时间点与对照受试者收集的光谱之间观察到统计学显著差异（p < 0.05 - 0.005）。我们首次证明了一氧化氮缺乏和高血压导致血浆生化谱的变化，并通过FTIR光谱检测到。
Mouse model of nitric oxide deficiency, induced by prolonged treatment with NG‐nitro‐L‐arginine methyl ester (L‐NAME) was used for infrared spectroscopy (FTIR) analysis of plasma. L‐NAME leads to increased peripheral resistance and systemic hypertension. Classification of spectral response was by principal component analysis (PCA) and linear discriminant analysis (LDA). PCA allowed to separate each animal group showing that FTIR spectra are sensitive to development of NO‐deficiency on contrary to blood pressure values indicating hypertension. Globally, the most pronounced spectral alternations were observed in the second and third week of L‐NAME treatment indicating that infrared signature of blood plasma can serve as indicator of early and late stages of the disease. The PLS‐DA method provided >95% classification accuracy. Spectral features characteristic for L‐NAME treatment were mainly associated with an elevated level of proteins accompanied by a decrease of a tyrosine content and changes in lipids/phospholipid concentration. In our work we discuss these changes for which statistically significant differences (p < 0.05 – 0.005) were observed between spectra collected for each time‐point of the L‐NAME treatment versus control subjects. We demonstrated for the first time that NO‐deficiency and hypertension resulted in changes in biochemical profile of plasma that was detected by FTIR spectroscopy.