Structures of monosialyl oligosaccharides isolated from the respiratory mucins of a non-secretor (O, Lea+b-) patient suffering from chronic bronchitis. Characterization of a novel type of mucin carbohydrate core structure.
Structures of monosialyl oligosaccharides isolated from the respiratory mucins of a non-secretor (O, Lea+b-) patient suffering from chronic bronchitis. Characterization of a novel type of mucin carbohydrate core structure.
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从患有慢性支气管炎的非分泌者（O，Lea b-）患者的呼吸道粘蛋白中分离出单唾液酸寡糖的结构。
DOI：
10.1093/glycob/4.2.203
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发表时间：
1994
期刊：
影响因子：
4.3
通讯作者：
Roussel,P
中科院分区：
文献类型：
--
作者：
vanHalbeek,H;Strang,AM;Lhermitte,M;Rahmoune,H;Lamblin,G;Roussel,P
从无分泌物型慢性支气管炎患者呼吸道粘液中制备粘蛋白糖肽类。低聚糖经碱性硼氢化物处理后释放，在硅键合烷基胺柱上采用阴离子交换层析、排粒径层析和高效液相层析进行纯化。结构研究采用500-MHz1H-NMR光谱和快速原子轰击-质谱法。从二糖到八糖大小的二十六种单胞寡糖，在本研究中得到了充分的表征。唾液酸发生在α(2←3)-或α(2←6)-与半乳糖残基相连，或α(2←6)-与半乳糖醇相连。与患者的非分泌状态保持一致，未发现具有α（1←2）连接的病灶残留物的结构。其中9个结构集中存在于α（1←3）连锁中，或在lex行列式上，或在sialyl- lex行列式上，或两者都存在（结构11）。只有一种低聚糖（结构3b）在leadet中与焦点α（1←4）连接。从非分泌物患者的黏蛋白中分离出的8个结构在分泌物个体的呼吸黏蛋白中未被发现；它们列在下面。其中，结构4α代表了一种新型粘蛋白碳水化合物核心结构。
Mucin glycopeptides were prepared from the respiratory mucus of a non-secretor, chronic bronchitis patient with blood group O, Lea+b−. Oligosaccharides were released by alkaline borohydride treatment and purified by anionexchange chromatography, size-exclusion chromatography and high-performance liquid chromatography on a silica-bonded alkylamine column. Structural studies employed 500-MHz1H-NMR spectroscopy and fast atom bombardment-mass spectrometry. Twenty-six monosialyl oligosaccharides ranging in size from di- to octasaccharide, were fully characterized in this study. The sialic acid occurs either α(2←3)- or α(2←6)-linked to a galactosyl residue, or α(2←6)-linked to GalNAc-ol. In keeping with the nonsecretor status of the patient, no structures with an α(1←2)-linked fucose residue were found. Nine of the structures had fucose present in α(1←3) linkage, either in the Lexdeterminant or in the sialyl-Lexdeterminant, or both (structure 11). Only one oligosaccharide (structure 3b) was seen with fucose α(1←4)-linked in the Leadeterminant. Eight structures isolated from the mucins of the non-secretor patient had not been found previously in the respiratory mucins of secretor individuals; they are Listed below. Among these, structure 4α represents a novel type of mucin carbohydrate core structure.