PRIMARY CULTURES OF HUMAN BLOOD-BORNE MACROPHAGES GROWN ON HYDROPHOBIC TEFLON MEMBRANES
PRIMARY CULTURES OF HUMAN BLOOD-BORNE MACROPHAGES GROWN ON HYDROPHOBIC TEFLON MEMBRANES
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DOI：
10.1016/s0022-1759(83)80019-2
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发表时间：
1983-01-01
影响因子：
2.2
通讯作者：
LOHR, GW
中科院分区：
文献类型：
--
作者：
ANDREESEN, R;PICHT, J;LOHR, GW
使用人混合AB血清作为必需生长因子，在Teflon箔（氟化乙烯丙烯共聚物）的疏水侧上培养人血源性单核细胞。在培养的任何阶段，这些体外成熟的巨噬细胞可以容易地从Teflon膜上分离并进行进一步的实验。一旦建立，原代巨噬细胞培养物可以在含有10%FCS [胎牛血清]的培养基中维持长达3个月。在单核细胞转化为巨噬细胞的连续过程中，溶菌酶分泌增加了10倍以上，并与细胞数量和成熟阶段相关。抑制人永久性肿瘤细胞系生长的能力也在巨噬细胞成熟过程中发展。对22名健康供体细胞的研究显示，成熟巨噬细胞对[人白血病] K562和MOLT 4肿瘤细胞具有可重复的活性。该系统将促进对人类巨噬细胞分化和功能的各个方面的研究。
Human blood-borne monocytes were cultivated on the hydrophobic side of Teflon foils (fluorinated ethylene propylene copolymer) using human pooled AB-serum as essential growth factor. At any stage of culture these in vitro maturing macrophages can easily be detached from the Teflon membrane and subjected to further experimentation. Once established, primary macrophage cultures can be maintained in medium containing 10% FCS [fetal calf serum] for up to 3 mo. Lysozyme secretion increased more than 10-fold during the sequential process of monocyte transformation into macrophages and correlates with cell number and stage of maturation. The ability to inhibit growth of human permanent tumor cell lines also developed during macrophage maturation. Studies on the cells of 22 healthy donors revealed a reproducible activity of mature macrophages against [human leukemia] K562 and MOLT4 tumor cells. The system will facilitate investigations on various aspects of human macrophage differentiation and function.