Pressurized fluid extraction of carotenoids from Haematococcus pluvialis and Dunaliella salina and kavalactones from Piper methysticum
Pressurized fluid extraction of carotenoids from Haematococcus pluvialis and Dunaliella salina and kavalactones from Piper methysticum
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DOI：
10.1016/j.aca.2003.09.026
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发表时间：
2004-01-16
影响因子：
6.2
通讯作者：
Li, QX
中科院分区：
文献类型：
--
作者：
Denery, JR;Dragull, K;Li, QX
研究了加压流体萃取法（PFE）提取绿色海藻雨生红球藻（Haematococcus pluvialis）和盐生杜氏藻（Dunaliella salina）中类胡萝卜素以及卡瓦胡椒（Piper methysticum）中卡法根内酯的工艺。通过改变提取溶剂、料液比、提取温度和提取时间等关键因素，对提取工艺进行优化。采用高效液相色谱（LC）和液质联用（LC-MS）确定提取参数的选择性和效率。结果表明，在无氧和无光的环境中，在受控的温度和压力下，PFE利用常规溶剂可以在更短的时间内使用更少的溶剂。与传统的溶剂萃取相比，PFE表现出更高或相等的萃取效率，同时保持化学成分的完整性。PFE在天然产物和营养保健品的提取方面表现出很高的潜力，特别是不稳定和光敏化学品。(C)2003 Elsevier B. V.保留所有权利。
Pressurized fluid extraction (PFE) was examined as an alternative technology for the extraction of carotenoids in the green algae Haematococcus pluvialis and Dunaliella salina and kavalactones in Piper methysticum. The extraction process was optimized by varying the key extraction factors of solvent, sample-solvent ratio, temperature, and time. The selectivity and efficiency of extraction parameters were determined with high performance liquid chromatography (LC) and LC-mass spectrometry (LC-MS). Results showed that PFE utilization of conventional solvents under controlled temperature and pressure in an oxygen and light-free environment could result in the use of less solvent in a shorter period of time. PFE showed higher or equal extraction efficiencies as compared with traditional solvent extractions while maintaining the integrity of chemical components. PFE showed high potential for extraction of natural products and nutraceuticals, particularly labile and light sensitive chemicals. (C) 2003 Elsevier B.V. All rights reserved.