FORM AND FUNCTION OF GLYCOCALYX ON FREE CELL-SURFACES
FORM AND FUNCTION OF GLYCOCALYX ON FREE CELL-SURFACES
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DOI：
10.1098/rstb.1974.0015
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发表时间：
1974-01-01
期刊：
影响因子：
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通讯作者：
ITO, S
中科院分区：
文献类型：
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作者：
ITO, S
本文列举了立克次体、细菌、变形虫、海胆卵和猫肠微绒毛上的糖萼或细胞壁，并讨论了它们的功能作用。注意到各种表面涂层形式的差异；虽然许多表面成分实际上是无关的消耗性涂层，但其他成分则是如此牢固地附着在细胞上，似乎是细胞的永久组成部分。本文对猫肠微绒毛的绒毛表面进行了较为详细的研究，并对糖萼的形态进行了一些新的观察。在甘油组织的断裂表面复制品中，肠道表面涂层不容易被观察到，但冷冻蚀刻后复制的固定细胞在蒸馏水中冷冻时，可以看到附着在微绒毛上的丝状网状结构的华丽阵列。与薄切片材料相比，这种模糊涂层在冻蚀制剂中至少厚两倍。未经固定或甘油处理而冷冻的新鲜组织没有一层厚厚的绒毛。在它的位置上发现了一层薄薄的无定形毯子状层。
Selected examples of the glycocalyx or cell coat on rickettsiae, bacteria, amoebae, sea-urchin eggs and the cat intestinal microvilli are illustrated and their functional roles are discussed. The differences in the form of various surface coats are noted; while many surface components are truely extraneous expendable coatings, others are so firmly attached that they seem to be a permanent part of the cell. The fuzzy surface coat on the cat intestinal microvilli have been considered in some detail and some new observations on the form of the glycocalyx are presented. The enteric surface coat is not readily visualized in fractured surface replicas of glycerinated tissue but fixed cells frozen in distilled water when replicated after freeze-etching reveal a flamboyant array of a filamentous meshwork attached to the microvilli. This fuzzy coat layer is at least twice as thick in the freeze-etched preparations when compared to thin sectioned material. Fresh tissue frozen without fixation or glycerin treatment did not have a thick fuzzy coat. In its place a thin amorphous blanket-like layer was found.