Micron-scale geometrical features of microtubules as regulators of microtubule organization.
Micron-scale geometrical features of microtubules as regulators of microtubule organization.
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DOI：
10.7554/elife.63880
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发表时间：
2021-06-11
期刊：
影响因子：
7.7
通讯作者：
Subramanian R
中科院分区：
文献类型：
--
作者：
Mani N;Wijeratne SS;Subramanian R
从单个微管中组织微米大小的多微管阵列对于不同的细胞功能是必不可少的。微管聚合物在很大程度上被视为组织过程中的被动构建块。一个例外是“微管蛋白代码”，在氨基酸水平上对微管蛋白的改变会影响微管相关蛋白的活性。最近的研究表明，单个微管和聚合物网络的微米尺度几何特征，如微管长度、重叠长度、接触角和晶格缺陷，也可以调节微管相关蛋白的活性和调节聚合物动力学。我们讨论了微管晶格的这种几何特性与相关蛋白质的活性之间的相互作用如何直接影响阵列组织的多个方面，从微管成核和排列到阵列尺寸的规格和动态网络的重塑。本文综述的机制强调了微管的微米尺寸特征是微管自组织研究中需要常规研究的关键参数。
The organization of micron-sized, multi-microtubule arrays from individual microtubules is essential for diverse cellular functions. The microtubule polymer is largely viewed as a passive building block during the organization process. An exception is the ‘tubulin code’ where alterations to tubulin at the amino acid level can influence the activity of microtubule-associated proteins. Recent studies reveal that micron-scale geometrical features of individual microtubules and polymer networks, such as microtubule length, overlap length, contact angle, and lattice defects, can also regulate the activity of microtubule-associated proteins and modulate polymer dynamics. We discuss how the interplay between such geometrical properties of the microtubule lattice and the activity of associated proteins direct multiple aspects of array organization, from microtubule nucleation and coalignment to specification of array dimensions and remodeling of dynamic networks. The mechanisms reviewed here highlight micron-sized features of microtubules as critical parameters to be routinely investigated in the study of microtubule self-organization.