Self-regulated left-right asymmetric expression of Pitx2c in the developing limb
Self-regulated left-right asymmetric expression of Pitx2c in the developing limb
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Pitx2c 在发育肢体中的自我调节左右不对称表达
DOI：
10.1016/j.ydbio.2014.09.002
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
H.
中科院分区：
文献类型：
--
作者：
Shiratori;H.;Yashiro;Iwai;N.;Oki;S.;Ikawa;Y.;Kanata;K.;and Hamada;H.
转录因子 Pitx2c 在原始内脏器官中以左右 (L-R) 不对称方式表达，并执行部位特异性形态发生。在这里，我们表明 Pitx2cis 在发育中的小鼠肢体中也 L-R 不对称表达。 HumanPITX2c 在小鼠肢体中表现出相同的转录活性。 Pitx2在肢体中的不对称表达也表现出背腹和前后极性，仅限于左肢的后背区域。肢体中的左侧 Pitx2c 表达受到 Nodal 信号通过 Nodal 响应增强子的调节。Pitx2cis 在左侧肢体的侧板中胚层 (LPM) 衍生细胞中表达，这些细胞有助于形成各种肢体结缔组织。发现左肢 Pitx2c+ 细胞的数量受到 Pitx2c 本身的负调节。虽然缺乏不对称Pitx2c表达的小鼠的肢体没有明显的缺陷，但Pitx2c可能调节肢体功能性L-R不对称性。
The transcription factor Pitx2c is expressed in primordial visceral organs in a left-right (L-R) asymmetric manner and executes situs-specific morphogenesis. Here we show thatPitx2cis also L-R asymmetrically expressed in the developing mouse limb. HumanPITX2cexhibits the same transcriptional activity in the mouse limb. The asymmetric expression ofPitx2cin the limb also exhibits dorsal-ventral and anterior-posterior polarities, being confined to the posterior-dorsal region of the left limb. Left-sidedPitx2cexpression in the limb is regulated by Nodal signaling through a Nodal-responsive enhancer.Pitx2cis expressed in lateral plate mesoderm (LPM)–derived cells in the left limb that contribute to various limb connective tissues. The number of Pitx2c+cells in the left limb was found to be negatively regulated by Pitx2c itself. Although obvious defects were not apparent in the limb of mice lacking asymmetricPitx2cexpression, Pitx2c may regulate functional L-R asymmetry of the limb.