Time-lapse electrical recordings of single neurons from the mouse neocortex
Time-lapse electrical recordings of single neurons from the mouse neocortex
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DOI：
10.1073/pnas.1214434110
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发表时间：
2013-04-02
影响因子：
11.1
通讯作者：
Mizrahi, Adi
中科院分区：
文献类型：
--
作者：
Cohen, Lior;Koffman, Noa;Mizrahi, Adi
大脑适应环境需求的能力意味着神经元可以在一生中发生变化。然而，单个神经元实际变化的程度在很大程度上仍未得到研究。为了评估单个神经元的功能特性如何随时间变化，我们设计了一种从完全相同的神经元进行体内延时电生理记录的方法。我们监测了小鼠体感皮层单个 L2/3 神经元的对侧和同侧感觉诱发尖峰活动。在第一次记录结束时，我们用 DNA 质粒电穿孔神经元以驱动 GFP 表达。然后，2周后，我们第二次目视引导体内记录电极到达表达GFP的神经元。我们发现，对侧和同侧诱发反应（即反应概率、潜伏期和偏好）以及单个 L2/3 锥体神经元的自发活动在控制条件下是稳定的，但这种稳定性可能会迅速被破坏。对侧胡须剥夺引起单个神经元的感觉诱发反应谱的剧烈变化。我们的实验提供了一个利用电生理学长期研究单个神经元的稳定性和可塑性的框架。
The ability of the brain to adapt to environmental demands implies that neurons can change throughout life. The extent to which single neurons actually change remains largely unstudied, however. To evaluate how functional properties of single neurons change over time, we devised a way to perform in vivo time-lapse electrophysiological recordings from the exact same neuron. We monitored the contralateral and ipsilateral sensory-evoked spiking activity of individual L2/3 neurons from the somatosensory cortex of mice. At the end of the first recording session, we electroporated the neuron with a DNA plasmid to drive GFP expression. Then, 2 wk later, we visually guided a recording electrode in vivo to the GFP-expressing neuron for the second time. We found that contralateral and ipsilateral evoked responses (i.e., probability to respond, latency, and preference), and spontaneous activity of individual L2/3 pyramidal neurons are stable under control conditions, but that this stability could be rapidly disrupted. Contralateral whisker deprivation induced robust changes in sensory-evoked response profiles of single neurons. Our experiments provide a framework for studying the stability and plasticity of single neurons over long time scales using electrophysiology.