Secretion of IFN-gamma, IL-6, granulocyte-macrophage colony-stimulating factor and IL-10 cytokines after activation of human purified T lymphocytes upon CD38 ligation
Secretion of IFN-gamma, IL-6, granulocyte-macrophage colony-stimulating factor and IL-10 cytokines after activation of human purified T lymphocytes upon CD38 ligation
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DOI：
10.1006/cimm.1996.0267
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发表时间：
1996-11-01
影响因子：
4.3
通讯作者：
Malavasi, F
中科院分区：
文献类型：
--
作者：
Ausiello, CM;laSala, A;Malavasi, F
人CD 38是一种由不同免疫活性细胞表达的表面糖蛋白，与不同的跨膜信号分子相关，并在钙动员化合物环ADP-核糖的合成中起关键作用。本研究报告了纯化的外周血T细胞中的CD 38连接层特异性单克隆抗体（mAb）随后分泌离散的细胞因子。IL-6、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、IFN-γ和IL-10 mRNA表达是恒定的结果。在所有研究对象的CD 38活化的T淋巴细胞培养物中也检测到低水平的IL-2 mRNA，在大多数CD 38活化的T培养物中检测到低水平的IL-4和IL-5 mRNA。此外，CD 38介导的细胞因子诱导不需要T细胞增殖或添加抗原呈递细胞，总之，人CD 38在纯化的T细胞中运行活化途径，其通过诱导Th 1或Th 2细胞共有的细胞因子谱来起作用。(C)出版社：Academic Press，Inc.
Human CD38, a surface glycoprotein expressed by different immunocompetent cells, is associated with distinct transmembrane signaling molecules and plays a key role in the synthesis of cyclic ADP-ribose, a calcium-mobilizing compound. This study reports that CD38 ligation lay specific monoclonal antibodies (mAb) in purified peripheral blood T cells is followed by secretion of discrete cytokines. IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), IFN-gamma, and IL-10 mRNA expression were constant findings. Low levels of IL-2 mRNA were also detected in CD38-activated T lymphocyte cultures of all subjects studied, Low levels of IL-4 and IL-5 mRNA were detected in the majority of CD38-activated T cultures. Moreover, CD38 mediated cytokine induction does not require T cell proliferation or the addition of antigen presenting cells, in conclusion, human CD38 runs an activation pathway in purified T cells which operates through the induction of a cytokine profile shared by Th1 or Th2 cells. (C) 1996 Academic Press, Inc.