Antimicrobial action of achacin is mediated by L-amino acid oxidase activity
Antimicrobial action of achacin is mediated by L-amino acid oxidase activity
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DOI：
10.1016/s0014-5793(02)03608-6
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发表时间：
2002-11-20
期刊：
影响因子：
3.5
通讯作者：
Tsuchiya, T
中科院分区：
文献类型：
--
作者：
Ehara, T;Kitajima, S;Tsuchiya, T
Achacin是从褐云玛瑙螺（AchatinafulicaRrussac）粘液中分离纯化的一种抗菌糖蛋白，具有抗不道德防御作用。我们发现，achacin具有L-氨基酸氧化酶活性，可以产生细胞毒性H2 O2，但H2 O2的浓度不足以杀死细菌。不同的H_2O_2清除剂均能抑制阿查辛的抗菌活性。免疫化学分析表明，乙酰胆碱酯酶优先结合生长期细菌，占生长期依赖的抗菌活性的乙酰胆碱酯酶的重要作用。Achacin可能是一种重要的防御分子。(C)2002年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
Achacin is an antibacterial glycoprotein purified from the mucus of the giant snail, Achatina fulica Rrussac, as a Immoral defense factor. We showed that achacin has L-amino acid oxidase activity and can generate cytotoxic H2O2; however, the concentration of H2O2 was not sufficient to kill bacteria. The antibacterial activity of achacin was inhibited by various H2O2 scavengers. Immunochemical analysis revealed that achacin was preferentially bound to growth-phase bacteria, accounting for the important role in growth-phase-dependent antibacterial activity of achacin. Achacin may act as an important defense molecule against invading bacteria. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.