Novel bifunctional lysozyme-dextran conjugate that acts on both gram-negative and gram-positive bacteria
Novel bifunctional lysozyme-dextran conjugate that acts on both gram-negative and gram-positive bacteria
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新型双功能溶菌酶-葡聚糖缀合物，对革兰氏阴性和革兰氏阳性细菌均起作用
DOI：
10.1271/bbb1961.54.3057
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Kunihiko Kobayashi
中科院分区：
文献类型：
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作者：
Soichiro Nakamura;A. Kato;Kunihiko Kobayashi
人们已经进行了许多尝试，以开发具有抗菌作用而没有任何食品毒性的食品防腐剂。其中最有前途的试剂之一是溶酶，如蛋清溶菌酶。溶菌酶只攻击细菌细胞壁中肽聚糖层的乙酰己糖胺之间的糖苷键的特定位置。革兰氏阳性细胞的细胞壁通常对溶菌酶的攻击相当敏感。然而，革兰氏阴性菌不太容易受到这种攻击，因为外膜排斥溶菌酶并阻止它与细胞壁上肽聚糖的作用位点结合。表面活性剂可溶解主要由脂多糖组成的外膜。因此，如果溶菌酶转化为两亲性蛋白，它可能会攻击革兰氏阴性菌。我们已经报道过，通过自然发生的美拉德反应得到的卵清蛋白-葡聚糖缀合物具有优于商品乳化剂的优异乳化性能。引人注目的是，以类似方式获得的溶菌酶-葡聚糖缀合物比卵清蛋白-葡聚糖缀合物表现出更大的乳化特性。此外，水解活性
Manyattempts have been made to develop food preservatives with antimicrobial effects without any food toxicities.1'2* One of the most promising reagents is lytic enzymes such as egg white lysozyme. Lysozyme attacks only specific positions of glycosidic bonds between Nacetylhexosamines of the peptidoglycan layer in bacterial cell walls. The walls of Grampositive cells are generally quite sensitive to attack by lysozyme. However, Gram-negative bacteria are less susceptible to such attack, since the outer membraneexcludes lysozyme and prevents it from binding to its site of action on the peptidoglycan in cell walls. Surfactants solubilize outer membranesmainly consisting of lipopolysaccharide.3 "6) Therefore, if lysozyme is converted to an amphiphilic protein, it may attack to Gram-negative bacteria. Wehave reported7) that ovalbumin-dextran conjugate obtained by the naturally occurring Maillard reaction has excellent emulsifying properties superior to commercial emulsifiers. Strikingly, lysozyme-dextran conjugate obtained in a similar way showed greater emulsifying properties than ovalbumin-dextran conjugate. In addition, the lytic activity