Ethylene promotes root hair growth through coordinated EIN3/EIL1 and RHD6/RSL1 activity in Arabidopsis
Ethylene promotes root hair growth through coordinated EIN3/EIL1 and RHD6/RSL1 activity in Arabidopsis
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DOI：
10.1073/pnas.1711723115
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发表时间：
2017-12-26
影响因子：
11.1
通讯作者：
Guo, Hongwei
中科院分区：
文献类型：
--
作者：
Feng, Ying;Xu, Ping;Guo, Hongwei
根毛是根表皮细胞的一种广泛结构，在无柄植物的养分获取、土壤锚定和环境相互作用中至关重要。植物激素乙烯（ET）促进根毛生长，也介导刺激毛细胞发育的不同信号的作用。然而，ET诱导根毛生长的分子基础仍然知之甚少。在这里，我们发现ET激活的转录因子乙烯不敏感3（EIN 3）与根毛缺陷6（RHD 6）（一种有充分证据的毛细胞正调节因子）物理相互作用，并且这两种因子直接共同激活头发长度决定基因RHD 6-LIKE 4（RSL 4）以促进根毛伸长。转录组分析进一步揭示了调节子对EIN 3/EIL 1（EIN 3-LIKE 1）和RHD 6/RSL 1（RHD 6-LIKE 1）的平行作用。EIN 3/EIL 1和RHD 6/RSL 1通过选择性调节核心根毛基因的一个子集协同增强根毛起始。因此，我们的工作揭示了一个关键的转录复合体组成的EIN 3/EIL 1和RHD 6/RSL 1在根毛的起始和伸长的控制，并提供了一个分子框架，整合环境信号和内在调节因子调节植物器官发育。
Root hairs are an extensive structure of root epidermal cells and are critical for nutrient acquisition, soil anchorage, and environmental interactions in sessile plants. The phytohormone ethylene (ET) promotes root hair growth and also mediates the effects of different signals that stimulate hair cell development. However, the molecular basis of ET-induced root hair growth remains poorly understood. Here, we show that ET-activated transcription factor ETHYLENE-INSENSITIVE 3 (EIN3) physically interacts with ROOT HAIR DEFECTIVE 6 (RHD6), a well-documented positive regulator of hair cells, and that the two factors directly coactivate the hair length-determining gene RHD6-LIKE 4 (RSL4) to promote root hair elongation. Transcriptome analysis further revealed the parallel roles of the regulator pairs EIN3/EIL1 (EIN3-LIKE 1) and RHD6/RSL1 (RHD6-LIKE 1). EIN3/EIL1 and RHD6/RSL1 coordinately enhance root hair initiation by selectively regulating a subset of core root hair genes. Thus, our work reveals a key transcriptional complex consisting of EIN3/EIL1 and RHD6/RSL1 in the control of root hair initiation and elongation, and provides a molecular framework for the integration of environmental signals and intrinsic regulators in modulating plant organ development.