SUBCELLULAR-DISTRIBUTION OF SHEAR-STRESS AT THE SURFACE OF FLOW-ALIGNED AND NONALIGNED ENDOTHELIAL MONOLAYERS
SUBCELLULAR-DISTRIBUTION OF SHEAR-STRESS AT THE SURFACE OF FLOW-ALIGNED AND NONALIGNED ENDOTHELIAL MONOLAYERS
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DOI：
10.1152/ajpheart.1995.268.4.h1765
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发表时间：
1995-04-01
影响因子：
4.8
通讯作者：
DAVIES, PF
中科院分区：
文献类型：
--
作者：
BARBEE, KA;MUNDEL, T;DAVIES, PF
在亚细胞尺度上测定了剪切流作用于内皮细胞腔表面的应力。使用原子力显微镜测量在无流动条件下或暴露于稳定剪切应力（12 dyn/cm（2）24 h）下培养的汇合内皮单层的表面形貌。通过计算流体力学模拟了这些表面几何形状上的流动，并计算了细胞表面上的剪切应力分布。流动扰动由于起伏的表面产生细胞尺度的变化的剪切应力大小，因此大的剪切应力梯度。重组的内皮细胞表面的反应，长期暴露于稳定的流导致显着减少的峰值剪切应力和剪切应力梯度。根据表面几何形状和由此产生的剪应力分布之间的关系，我们定义了一个流体动力学形状因子，该因子表征了内皮细胞对流动的三维形态反应。该分析提供了一个完整的描述的空间分布的应力对单个内皮细胞内的一个融合的单层规模相关的机械转导的物理机制的研究。
The stresses acting on the luminal surface of endothelial cells due to shear flow were determined on a subcellular scale. Atomic force microscopy was used to measure the surface topography of confluent endothelial monolayers cultured under no-flow conditions or exposed to steady shear stress (12 dyn/cm(2) for 24 h). Flow over these surface geometries was simulated by computational fluid dynamics, and the distribution of shear stress on the cell surface was calculated. Flow perturbations due to the undulating surface produced cell-scale variations of shear stress magnitude and hence large shear stress gradients. Reorganization of the endothelial surface in response to prolonged exposure to steady flow resulted in significant reductions in the peak shear stresses and shear stress gradients. From the relationship between surface geometry and the resulting shear stress distribution, we have defined a hydrodynamic shape factor that characterizes the three-dimensional morphological response of endothelial cells to flow. The analysis provides a complete description of the spatial distribution of stresses on individual endothelial cells within a confluent monolayer on a scale relevant to the study of physical mechanisms of mechano-transduction.