A new wc-1 mutant of Neurospora crassa shows unique light sensitivity in the circadian conidiation rhythm
A new wc-1 mutant of Neurospora crassa shows unique light sensitivity in the circadian conidiation rhythm
复制标题
粗糙脉孢菌的一种新的 wc-1 突变体在昼夜节律中表现出独特的光敏感性
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
H. Nakashima
中科院分区：
文献类型：
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作者：
K. Toyota;K. Onai;H. Nakashima
抽象的。利用含有粗糙脉孢菌（Neurospora crassa）基因组中cmd基因上游区域的质粒，通过DNA插入诱变，分离到一个新的时钟突变体（rhy-2）。该突变体在连续黑暗条件下分生孢子的形成是无节律的，但在光暗循环条件下分生孢子的形成是有节律的。从rhy-2菌株的基因组DNA中拯救质粒后，将插入定位于基因白色领-1（wc-1）。将质粒DNA插入到编码WC-1基因产物的多聚谷氨酰胺区域的序列的3′端，并且编码截短的WC-1蛋白的mRNA必须在cmd启动子的控制下合成。新的wc-1突变体rhy-2仍然对光敏感，尽管只是微弱的。由于连续黑暗中的分生孢子的昼夜节律被消除的突变体，在WC-1的多聚谷氨酰胺区域可能是必不可少的时钟功能和光诱导的胡萝卜素在脉孢菌。
Abstract. A new clock mutant (rhy-2) was isolated by DNA insertion mutagenesis using a plasmid that contains a region located upstream of the cmd gene in the genome of Neurospora crassa. This mutant is arrhythmic with regard to conidiation in continuous darkness but rhythmic under a light-dark cycle. After plasmid rescue from genomic DNA of the rhy-2 strain, the insertion was localized to the gene white collar-1 (wc-1). Plasmid DNA was inserted 3′ to the sequence encoding the polyglutamine region of the WC-1 gene product, and an mRNA encoding a truncated WC-1 protein must be synthesized under the control of the cmd promoter. The new wc-1 mutant, rhy-2, is still sensitive to light, although only weakly. Since the circadian rhythm of conidiation in continuous darkness is eliminated in the mutant, the polyglutamine region in WC-1 may be essential for both clock function and light-induced carotenogenesis in Neurospora.
影响因子：
56.9
作者：
Crosthwaite, SK;Dunlap, JC;Loros, JJ
通讯作者：
Loros, JJ
影响因子：
56.9
作者：
Lee, K;Loros, JJ;Dunlap, JC
通讯作者：
Dunlap, JC