Mechanisms generating dual-component nicotinic EPSCs in cortical interneurons.
Mechanisms generating dual-component nicotinic EPSCs in cortical interneurons.
复制标题
在皮质中间神经元中产生双组分烟碱EPSC的机制。
DOI：
10.1523/jneurosci.3565-12.2012
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发表时间：
2012-11-28
期刊：
影响因子：
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通讯作者：
Hestrin S
中科院分区：
文献类型：
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作者：
Bennett C;Arroyo S;Berns D;Hestrin S
基底前脑 (BF) 胆碱能轴突激活皮质烟碱受体会显着影响皮质功能，而烟碱受体的丧失是衰老和神经退行性疾病的标志。我们之前已经表明，刺激 BF 轴突会在皮质中间神经元中产生快速的 α7 和缓慢的非 α7 受体依赖性反应。然而，这种双成分烟碱反应背后的突触机制仍不清楚。在此，我们报道小鼠皮质中快速α7受体介导的EPSC高度可变且对乙酰胆碱酯酶（AChE）的扰动不敏感，而缓慢的非α7受体介导的EPSC可靠且对AChE活性高度敏感。基于这些数据，我们提出快速和慢速烟碱反应反映了激活α7和非α7类烟碱受体的胆碱能静脉曲张之间突触结构的差异。
Activation of cortical nicotinic receptors by cholinergic axons from the basal forebrain (BF) significantly impacts cortical function, and the loss of nicotinic receptors is a hallmark of aging and neurodegenerative disease. We have previously shown that stimulation of BF axons generates a fast α7 and a slow non-α7 receptor-dependent response in cortical interneurons. However, the synaptic mechanisms that underlie this dual-component nicotinic response remain unclear. Here, we report that fast α7 receptor-mediated EPSCs in the mouse cortex are highly variable and insensitive to perturbations of acetylcholinesterase (AChE), while slow non-α7 receptor-mediated EPSCs are reliable and highly sensitive to AChE activity. Based on these data, we propose that the fast and slow nicotinic responses reflect differences in synaptic structure between cholinergic varicosities activating α7 and non-α7 classes of nicotinic receptors.