eDNA biomonitoring revealed the ecological effects of water diversion projects between Yangtze River and Tai Lake.
eDNA biomonitoring revealed the ecological effects of water diversion projects between Yangtze River and Tai Lake.
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DOI：
10.1016/j.watres.2021.117994
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发表时间：
2021-12
期刊：
影响因子：
12.8
通讯作者：
Lijuan Zhang;Jianghu Yang;Yong Zhang;Jun-zhe Shi;Hongxia Yu;Xiaowei Zhang
中科院分区：
文献类型：
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作者：
Lijuan Zhang;Jianghu Yang;Yong Zhang;Jun-zhe Shi;Hongxia Yu;Xiaowei Zhang
调水已被广泛用于解决水资源短缺和安全问题。然而，由于常规生物监测的局限性，其长期的生态影响，特别是对当地社区的生物多样性和结构的影响，往往被忽视。以引江济太工程为例，采用环境DNA(EDNA)元生物编码方法，研究了引水工程对流域的潜在生态影响。首先，通过16S rDNA V3区和18S rDNA V9区的元编码，从26个测点中鉴定出136个浮游植物属/种，其中蓝藻31个，真核浮游植物105个。与形态学方法相比，EDNA Metabarding在检测纳米/微微浮游生物(<；20μm)方面显示出明显的优势。其次，与太湖相连的贡湖湾/竹山湾与太湖中心相比，群落组成的相似性和共享度较高，说明引水作用加速了不同水体之间的生物同质化。在太湖不同连通区域(占真浮游植物总数的0.03%~0.54%)检测到长江本地种(占真浮游植物总数的0.03%~0.54%)，其相对丰度与长江引水的影响一致。此外，OFS的引入。钾肥对太湖浮游植物群落组成有显著影响。西引黄工程对太湖西部浮游植物的生态效应(如类群丰富度、群落组成和物种入侵)明显高于东引黄工程，这与引水工程的规模(水量和历时)相一致。总体而言，本研究强调了EDNA生物监测在调水工程生态影响评估中的价值。
Water diversion has been widely used to address water shortages and security issues. However, its long-term ecological impacts, particularly on the biodiversity and structure of the local community, have often been neglected due to limitations of conventional biomonitoring. Taking the water diversion projects from Yangtze River to Tai Lake (WDYT) as examples, environmental DNA (eDNA) metabarcoding was used to investigate the potential ecological impact of water diversion on the connected basins. Firstly, 136 phytoplankton genera/species, including 31 cyanobacteria and 105 eukaryotic phytoplankton (Euk-phytoplankton), were identified from 26 sites by metabarcoding of 16S rDNA V3 and 18S rDNA V9 regions. eDNA metabarcoding showed an obvious advantage in detecting nano/pico-plankton (< 20 μm in size) compared with the morphological approach. Secondly, more shared taxa and higher similarity of community composition were observed in Gonghu Bay/Zhushan Bay with its connected river than with the center of Tai Lake, indicating that water diversions were accelerating the biotic homogenization between different waterbodies.Skeletonema potamos, the native species of Yangtze River (4.04% of the total Euk-phytoplankton reads) was detected in different connecting regions of Tai Lake (0.03%-0.54% of the total Euk-phytoplankton reads), where its relative abundance was consistent with the influence of water diversion from Yangtze River. Furthermore, the introduction ofS. potamossignificantly affected the local community compositions of phytoplankton in Tai Lake. Finally, the ecological effect (e.g., taxa richness, community composition and species invasion) of the WDYT on phytoplankton in the west of Tai Lake was more significant than that in the east, which was consistent with the scale (volume and duration) of the water diversion projects. Overall, this study highlights the value of eDNA biomonitoring in the ecological impact assessment of water transfer projects.