Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography
Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography
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DOI：
10.1117/1.2192804
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发表时间：
2006-03-01
影响因子：
3.5
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Wang, Xueding;Xie, Xueyi;Wang, Lihong V.
利用基于激光的光声断层扫描(PAT)技术，实现了活体大鼠脑内两个功能参数--血红蛋白总浓度和血氧饱和度的无创性经颅成像。就像在光学扩散光谱中一样，PAT可以评估内源性发色团在多个光学波长下的光学吸收，例如氧化和脱氧血红蛋白。然而，PAT可以提供高空间分辨率，因为它的分辨率受到光声信号的衍射限制，而不是光学扩散。连续使用两种波长的激光脉冲，通过完整的皮肤和头骨，获取大鼠大脑皮层血管系统的光声图像。以良好的空间分辨率，成功地显示了大脑皮质静脉血管内血容量和血氧含量的分布，这些变化受到了包括高氧、常氧和低氧在内的全身生理调节的影响。这项技术对内源性对比具有显著的敏感性，可能有助于理解大脑中神经、血流动力学和代谢活动之间的相互关系。(C)2006年光学仪器工程师学会。
Simultaneous transcranial imaging of two functional parameters, the total concentration of hemoglobin and the hemoglobin oxygen saturation, in the rat brain in vivo is realized noninvasively using laser-based photoacoustic tomography (PAT). As in optical diffusion spectroscopy, PAT can assess the optical absorption of endogenous chromophores, e. g., oxygenated and deoxygenated hemoglobins, at multiple optical wavelengths. However, PAT can provide high spatial resolution because its resolution is diffraction-limited by photoacoustic signals rather than by optical diffusion. Laser pulses at two wavelengths are used sequentially to acquire photoacoustic images of the vasculature in the cerebral cortex of a rat brain through the intact skin and skull. The distributions of blood volume and blood oxygenation in the cerebral cortical venous vessels, altered by systemic physiological modulations including hyperoxia, normoxia, and hypoxia, are visualized successfully with satisfactory spatial resolution. This technique, with its prominent sensitivity to endogenous contrast, can potentially contribute to the understanding of the interrelationship between neural, hemodynamic, and metabolic activities in the brain. (c) 2006 Society of Photo-Optical Instrumentation Engineers.