An In Planta Method for Assessing the Role of Basidiospores in Rhizoctonia Foliar Disease of Tomato
An In Planta Method for Assessing the Role of Basidiospores in Rhizoctonia Foliar Disease of Tomato
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DOI：
10.1094/pdis-94-5-0515
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发表时间：
2010-05-01
期刊：
影响因子：
4.5
通讯作者：
Ivors, Kelly L.
中科院分区：
文献类型：
--
作者：
Bartz, Faith E.;Cubeta, Marc A.;Ivors, Kelly L.
2005 - 2006年在北卡罗来纳州发生了一种番茄叶部枯萎病，症状包括坏死病变和枯萎叶片。叶片背面有白色菌丝生长的迹象。症状叶片上分离物的形态与Rluzoctoniasolani一致，因为症状表现模式表明担孢子湿润了初次接种源。利用在植物体内产生担孢子的方法实现了Koch的假设。分离物通过形态学表征。DNA序列分析菌丝融合。和体细胞菌丝相互作用系统发育分析和菌丝融合标准支持将分离物置于立枯丝核菌融合群3（AG-3）中。来自NC的番茄叶枯病分离物与立枯丝核菌的番茄分离物形成单一系统发育群：AG-3来自日本，与R solani AG-3个马铃薯分离物的菌丝互作比烟草分离物的菌丝互作强据我们所知，马铃薯分离物在葡萄酒中的菌丝互作既有亲和性，也有不亲和性。这是首次详细报道番茄叶枯病菌R. solani AG-3在北美全面描述了用于生产担孢子的技术，其潜在用途是了解其他Rluzoctonia病理系统中的叶病病因
A tomato (Solanum Ivcoperstcum) foliar blight disease of unknown etiology was observed in North Carolina (NC) dun on 2005 to 2006 Symptoms included necrotic lesions and blighted leaves. with signs of white mycelial growth on abaxial leaf surfaces The morphology of Isolates from symptomatic leaves was consistent with that of Rluzoctonia solani Because the pattern of symptom expression suggested that basidiospores wet e the primary inoculum source. Koch's postulates were fulfilled using a method to generate basidiospores In planta Isolates were characterized by morphology. DNA sequence analysis hyphal anastomosis. and somatic hyphal interactions Phylogenetic analyses and hyphal anastomosis criteria support placement of the isolates in R solani anastomosis group 3 (AG-3) Tomato foliar blight isolates from NC form a single phylogenetic group with tomato isolates of R solani: AG-3 from Japan and are more closely related to R solani AG-3 isolates from potato than tobacco Isolates exhibited both compatible and incompatible hyphal interactions when paired in vino To our knowledge. this is the first detailed report of tomato foliar blight caused by R. solani AG-3 in North America A comprehensive description of the technique employed for producing basidiospores is presented with potential utility for understanding foliar disease etiology in other Rluzoctonia pathosystems