EFFECT OF PROTEIN INGESTION ON SPLANCHNIC AND LEG METABOLISM IN NORMAL MAN IN PATIENTS WITH DIABETES-MELLITUS
EFFECT OF PROTEIN INGESTION ON SPLANCHNIC AND LEG METABOLISM IN NORMAL MAN IN PATIENTS WITH DIABETES-MELLITUS
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DOI：
10.1172/jci108375
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发表时间：
1976-01-01
影响因子：
15.9
通讯作者：
HAGENFELDT, L
中科院分区：
文献类型：
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作者：
WAHREN, J;FELIG, P;HAGENFELDT, L
在7名健康受试者和8名糖尿病患者中研究了富含蛋白质的膳食后底物的器官间通量。在基础状态和摄入瘦牛肉（3 g/kg体重）后3小时，检查了动脉浓度以及腿部和内脏氨基酸、碳水化合物底物、游离脂肪酸（FFA）和酮体的交换。糖尿病患者在研究前暂停胰岛素24小时。在正常受试者中，蛋白质摄入后，有大量的氨基酸从内脏床释放，主要涉及支链氨基酸。缬氨酸、异亮氨酸和亮氨酸一起占总内脏氨基酸产量的一半以上。在支链氨基酸（100-200%）的动脉浓度中观察到较大的增量，而其他氨基酸的增量较小。大多数氨基酸的腿交换从基础净输出恢复到蛋白质喂养后的净吸收，这是最显着的支链氨基酸。后者占总外周氨基酸摄取量的一半以上。
The inter-organ flux of substrates after a protein-rich meal was studied in seven healthy subjects and in eight patients, with diabetes mellitus. Arterial concentrations as well as leg and splanchnic exchange of amino acids, carbohydrate substrates, free fatty acids (FFA), and ketone bodies were examined in the basal state and for 3 h after the ingestion of lean beef (3 g/kg body wt). Insulin was withheld for 24 h before the study in the diabetic patients. In the normal subjects, after protein ingestion, there was a large amino acid release from the splanchnic bed predominantly involving the branched chain amino acids. Valine, isoleucine, and leucine accounted together for more than half of total splanchnic amino acid output. Large increments were seen in the arterial concentrations of the branched chain amino acids (100-200%) and to a smaller extent for other amino acids. Leg exchange of most amino acids reverted from a basal net outut to a net uptake after protein feeding which was most marked for the branched chain amino acids. The latter accounted for more than half of total peripheral amino acid uptake...