Bone marrow transplantation across major histocompatibility barriers in mice. II. T cell requirement for engraftment in total lymphoid irradiation-conditioned recipients.
Bone marrow transplantation across major histocompatibility barriers in mice. II. T cell requirement for engraftment in total lymphoid irradiation-conditioned recipients.
复制标题
跨越小鼠主要组织相容性障碍的骨髓移植。
DOI：
10.1097/00007890-198203000-00007
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发表时间：
1982
期刊：
影响因子：
6.2
通讯作者：
Kersey,JH
中科院分区：
文献类型：
--
作者：
Vallera,DA;Soderling,CC;Carlson,GJ;Kersey,JH
在移植前接受全淋巴细胞照射（TLI）的动物中，研究了T淋巴细胞在骨髓移植（BM）中的作用，这些动物跨越了主要的组织相容性障碍。供体骨髓（作为淋巴造血干细胞的来源）和脾脏细胞（作为移植物抗宿主病（GVHD）引起的细胞的来源）在体外用单克隆抗thy -1.2 +补体(C)预处理。然后将T细胞耗尽的移植物给予900 rad单剂量TLI加环磷酰胺（CY）的同种异体小鼠。这些小鼠没有移植。即使在没有添加脾脏细胞的情况下，与接受相同调节方案和未处理供体细胞的动物相比，消除供体骨髓移植物中的小T细胞群也阻止了移植物的移植。这些对照动物在移植后约1个月表现出一致的移植证据。当受者接受17 X 200 rad的TLI时，报告了类似的结果。
Studies were undertaken to examine the role of T lymphocytes in engraftment of bone marrow (BM) in animals conditioned with total lymphoid irradiation (TLI) prior to transplantation across major histocompatability barriers. Donor BM (added as a source of lymphohematopoietic stem cells) and spleen cells (added as a source of graft-versus-host disease (GVHD)-causing cells) were pretreated in vitro with monoclonal anti-Thy-1.2 plus complement (C). T cell-depleted grafts were then given to allogeneic mice conditioned with 900 rad of single dose TLI plus cyclophosphamide (CY). These mice did not engraft. Even in the absence of added spleen cells, elimination of the small T cell population from donor BM grafts prevented engraftment compared with animals that received the same conditioning regimen and untreated donor cells. These control animals demonstrated uniform evidence of engraftment about 1 month after transplantation. Similar findings were reported when recipients were conditioned with fractionated 17 X 200-rad TLI.