PRIMARY RESPONSES OF HUMAN T-CELLS TO MYCOBACTERIA - A FREQUENT SET OF GAMMA-DELTA T-CELLS ARE STIMULATED BY PROTEASE-RESISTANT LIGANDS
PRIMARY RESPONSES OF HUMAN T-CELLS TO MYCOBACTERIA - A FREQUENT SET OF GAMMA-DELTA T-CELLS ARE STIMULATED BY PROTEASE-RESISTANT LIGANDS
复制标题
DOI：
10.1002/eji.1830200534
复制
发表时间：
1990-05-01
影响因子：
5.4
通讯作者：
WAGNER, H
中科院分区：
文献类型：
--
作者：
PFEFFER, K;SCHOEL, B;WAGNER, H
表达T细胞受体α/β的T淋巴细胞亚群。β的或γ/。δ的从人外周血T细胞中选择，并测定对分子量分级的分枝杆菌裂解物的增殖反应。α/。β的T细胞主要应答于> 30 kDa的级分，而γ/.δ的T细胞优先与< 3 kDa的组分反应。蛋白酶消化消除了α/β的刺激活性。β的T细胞，证实了α/。β的T细胞对蛋白质成分有反应。相反，由γ/.δ的T细胞被证明对蛋白酶消化具有抗性。在有限稀释研究中，增殖的γ/β的频率为0.0001。δ的T细胞通过刺激性裂解物的蛋白酶处理几乎保持不变，而α/.β的T细胞几乎检测不到。此外，只有少数γ/。δ的T细胞对65 kDa的热休克蛋白有反应。我们的数据表明，与α/不同，β的T细胞，γ/。δ的T细胞对抵抗剧烈蛋白酶消化的分枝杆菌组分作出反应。
T lymphocyte subsets expressing either T cell receptor .alpha./.beta. or .gamma./.delta. were selected from human peripheral blood T cells and proliferative responses to molecular mass-fractionated mycobacterial lysates were determined. .alpha./.beta. T cells primarily responded to fractions > 30 kDa whereas .gamma./.delta. T cells preferentially reacted to fractions < 3 kDa. Protease digestion abolished the stimulating activities for .alpha./.beta. T cells, confirming the .alpha./.beta. T cells respond to protein components. In contrast, components recognized by .gamma./.delta. T cells proved resistant to protease digestion. In limiting dilution studies, frequencies of proliferating .gamma./.delta. T cells remained virtually unaltered by protease treatment of stimulating lysates, while those of .alpha./.beta. T cells became almost undetectable. Furthermore, only few .gamma./.delta. T cells responded to the 65-kDa heat-shock protein. Our data indicate that, unlike .alpha./.beta. T cells, .gamma./.delta. T cells respond to mycobacterial components which are resistant to vigorous protease digestion.