Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients
Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients
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DOI：
10.1016/j.biomaterials.2007.01.019
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发表时间：
2007-04-01
期刊：
影响因子：
14
通讯作者：
Spencer, Nicholas D.
中科院分区：
文献类型：
--
作者：
Kunzler, Tobias P.;Drobek, Tanja;Spencer, Nicholas D.
医用植入物的表面粗糙度非常重要，因为表面与宿主组织（例如骨、纤维组织）直接接触。细胞对粗糙度的响应取决于细胞类型而不同。然而，粗糙度对细胞行为的影响很少被系统地研究。我们已经开发了一种表面改性工艺，以产生覆盖一个基层上的粗糙度值范围很广的粗糙度梯度。这样的梯度允许对细胞行为的粗糙度的系统调查。使用两步粗糙化和平滑化工艺制造陶瓷，包括喷砂和随后的化学抛光步骤。为了产生一组相同的表面，我们应用了复制技术。用大鼠颅骨成骨细胞（RCO）和人牙龈成纤维细胞（HGF）进行细胞实验。RCO显示出显着增加的增殖率与增加表面粗糙度。在梯度上的不同位置，成骨细胞的足迹大小不同，在梯度的粗糙端保持较小，并随着粗糙度的降低而显著增加。HGF表现出相反的增殖行为，增殖随着粗糙度的增加而减少。发现成纤维细胞形态与成骨细胞相似。(c)2007爱思唯尔有限公司版权所有。
The surface roughness of a medical implant is of great importance since the surface is in direct contact with the host tissue (e.g. bone, fibrous tissue). The response of cells to roughness is different depending on the cell type. However, the influence of roughness on cell behavior has only rarely been systematically studied. We have developed a surface-modification process to produce roughness gradients that cover a wide range of roughness values on one substratum. Such gradients allow for systematic investigations of roughness on cell behavior. Gradients were fabricated using a two-step roughening and smoothening process, involving sandblasting and a subsequent chemical polishing step. In order to produce a set of identical surfaces we applied a replica technique. Cell experiments were carried out with rat calvarial osteoblasts (RCO) and human gingival fibroblasts (HGF). RCOs showed a significantly increased proliferation rate with increasing surface roughness. The footprint of osteoblasts varied in size at different positions on the gradient, remaining small on the rough end of the gradient and increasing considerably as the roughness decreased. HGF showed the opposite proliferation behavior, proliferation decreasing with increasing roughness. The fibroblast morphology was found to be similar to that seen for osteoblasts. (c) 2007 Elsevier Ltd. All rights reserved.