Matrix effect in bio-analysis of illicit drugs with LC-MS/MS: Influence of ionization type, sample preparation, and biofluid
Matrix effect in bio-analysis of illicit drugs with LC-MS/MS: Influence of ionization type, sample preparation, and biofluid
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DOI：
10.1016/s1044-0305(03)00574-9
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发表时间：
2003-11-01
影响因子：
3.2
通讯作者：
Murphy, CM
中科院分区：
文献类型：
--
作者：
Dams, R;Huestis, MA;Murphy, CM
本工作的目的是通过吗啡（10 杯/mL 溶液）柱后输注实验，评估电离类型、样品制备技术和生物流体对非法药物定量液相色谱 (LC)-MS/MS 分析中基质效应存在的协同效应。三种生物液体（尿液、口腔液和血浆）采用四种样品制备程序 [直接进样、稀释、蛋白质沉淀、固相萃取 (SPE)] 进行预处理，并通过 LC-电喷雾电离 (ESI)-MS/MS 和 LC-大气压化学电离 (APCI)-MS/MS 进行分析。我们的结果表明，两种电离类型均表现出基质效应，但 ESI 比 APCI 更容易受到影响。样品制备可以减少（净化）或放大（预浓缩）基质效应。残留基质成分对于每种生物液体是特异的，并且在色谱图中的不同时间点受到干扰。我们在方法开发的早期阶段评估了基质效应，并结合了每种生物液体的最佳电离类型和样品制备技术。简单稀释尿液就足以通过 LC-APCI-MS/MS 分析感兴趣的分析物。乙腈蛋白沉淀为口腔液分析提供了样品净化和浓缩，而 SPE 对于 LC-APCI-MS/MS 之前的血浆的广泛净化是必要的。 (C) 2003 年美国质谱学会。
The purpose of the present work was to evaluate the synergistic effect of ionization type, sample preparation technique, and bio-fluid on the presence of matrix effect in quantitative liquid chromatography (LC)-MS/MS analysis of illicit drugs by post-column infusion experiments with morphine (10-mug/mL solution). Three bio-fluids (urine, oral fluid, and plasma) were pretreated with four sample preparation procedures [direct injection, dilution, protein precipitation, solid-phase extraction (SPE)] and analyzed by both LC-electrospray ionization (ESI)-MS/MS and LC-atmospheric pressure chemical ionization (APCI)-MS/MS. Our results indicated that both ionization types showed matrix effect, but ESI was more susceptible than APCI. Sample preparation could reduce (clean up) or magnify (pre-concentrate) matrix effect. Residual matrix components were specific to each bio-fluid and interfered at different time points in the chromatogram. We evaluated matrix effect in an early stage of method development and combined optimal ionization type and sample preparation technique for each bio-fluid. Simple dilution of urine was sufficient to allow for the analysis of the analytes of interest by LC-APCI-MS/MS. Acetonitrile protein precipitation provided both sample clean up and concentration for oral fluid analysis, while SPE was necessary for extensive clean up of plasma prior to LC-APCI-MS/MS. (C) 2003 American Society for Mass Spectrometry.