QUANTITATION OF THE INVOLVEMENT OF THE RECA, RECB, RECC, RECF, RECJ, RECN, LEXA, RADA, RADB, UVRD, AND UMUC GENES IN THE REPAIR OF X-RAY-INDUCED DNA DOUBLE-STRAND BREAKS IN ESCHERICHIA-COLI
QUANTITATION OF THE INVOLVEMENT OF THE RECA, RECB, RECC, RECF, RECJ, RECN, LEXA, RADA, RADB, UVRD, AND UMUC GENES IN THE REPAIR OF X-RAY-INDUCED DNA DOUBLE-STRAND BREAKS IN ESCHERICHIA-COLI
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DOI：
10.2307/3576850
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发表时间：
1986-07-01
影响因子：
3.4
通讯作者：
SMITH, KC
中科院分区：
文献类型：
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作者：
SARGENTINI, NJ;SMITH, KC
携带单DNA修复突变的等基因大肠杆菌菌株进行了比较，比较了它们(i)使用中性蔗糖梯度测量的x射线诱导的DNA双链断裂（DSB）的修复能力；（ii）介质依赖电阻，即依赖reca的x射线存活现象，与修复DSB的能力密切相关；（iii）使用碱性蔗糖梯度测量x射线诱导的DNA单链断裂（SSB）的生长介质依赖性、reca依赖性修复（其中约80%的SSB实际上是DSB的一部分）。测量这三种能力是为了更准确地量化各种基因在大剂量范围内对DSB修复的参与。根据对x射线诱导的DSB修复的影响，将测试的突变分为5类：(1)recA、recB、recC和lexA突变体完全缺失；（II） radB和recN突变体缺失约90%；（III） recF和recJ突变体缺失约70%；(4) radA和uvrD突变体缺失约30%；(5) umuC突变体修复DSB的能力与野生型菌株相似。
Isogenic Escherichia coli strains carrying single DNA-repair mutations were compared for their capacity for (i) the repair of X-ray-induced DNA double-strand breaks (DSB) as measured using neutral sucrose gradients; (ii) medium-dependent resistance, i.e., recA-dependent X-ray survival phenomenon that correlates closely with the capacity for repairing DSB; and (iii) the growth medium-dependent, recA-dependent repair of X-ray-induced DNA single-strand breaks (SSB) as measured using alkaline sucrose gradients (about 80% of these SSB are actually parts of DSB). These three capacities were measured to quantitate more accurately the involvement of the various genes in the repair of DSB over a wide dose range. The mutations tested were grouped into five classes according to their effect on the repair of X-ray-induced DSB: (I) the recA, recB, recC, and lexA mutants were completely deficient; (II) the radB and recN mutants were about 90% deficient; (III) the recF and recJ mutants were about 70% deficient; (IV) the radA and uvrD mutants were about 30% deficient; and (V) the umuC mutants resembled the wild-type strains in it''s capacity for the repair of DSB.