Hypoxia-induced gene expression profiling in the euryoxic fish Gillichthys mirabilis
Hypoxia-induced gene expression profiling in the euryoxic fish Gillichthys mirabilis
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DOI：
10.1073/pnas.98.4.1993
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发表时间：
2001-02-13
影响因子：
11.1
通讯作者：
Somero, GN
中科院分区：
文献类型：
--
作者：
Gracey, AY;Troll, JV;Somero, GN
缺氧在生物医学和环境背景下都很重要，并且需要代谢组织的快速适应性变化。哺乳动物作为呼吸空气的动物，承受持续缺氧的能力有限。相比之下，一些水生动物，如某些鱼类，经常暴露在严重的环境缺氧和耐缺氧。了解缺氧胁迫下鱼类基因表达的变化，可以揭示新的耐受机制，可能为高等脊椎动物缺氧和缺血提供新的线索。利用cDNA微阵列技术，我们研究了耐缺氧的穴居虾虎鱼Gillichthys mirabilis的基因表达。我们表明，一个复杂的转录反应的连贯的图片可以生成一个非模式生物序列数据不可用。我们证明：（i）虽然某些基因表达的变化反映了哺乳动物的变化，但新基因在鱼类中的表达存在差异;（ii）组织特异性表达模式反映了组织在缺氧期间的不同代谢作用。
Hypoxia is important in both biomedical and environmental contexts and necessitates rapid adaptive changes in metabolic organization. Mammals, as air breathers, have a limited capacity to withstand sustained exposure to hypoxia. By contrast, some aquatic animals, such as certain fishes, are routinely exposed and resistant to severe environmental hypoxia. Understanding the changes in gene expression in fishes exposed to hypoxic stress could reveal novel mechanisms of tolerance that may shed new light on hypoxia and ischemia in higher vertebrates. Using cDNA microarrays, we have studied gene expression in a hypoxia-tolerant burrow-dwelling goby fish, Gillichthys mirabilis. We show that a coherent picture of a complex transcriptional response can be generated for a nonmodel organism for which sequence data were unavailable. We demonstrate that: (i) although certain shifts in gene expression mirror changes in mammals, novel genes are differentially expressed in fish; and (ii) tissue-specific patterns of expression reflect the different metabolic roles of tissues during hypoxia.