Tissue eosinophilia in acute and chronic atopic dermatitis: a morphometric approach using quantitative image analysis of immunostaining
Tissue eosinophilia in acute and chronic atopic dermatitis: a morphometric approach using quantitative image analysis of immunostaining
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DOI：
10.1046/j.1365-2133.2001.04456.x
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发表时间：
2001-11-01
影响因子：
10.3
通讯作者：
Kapp, A
中科院分区：
文献类型：
--
作者：
Kiehl, P;Falkenberg, K;Kapp, A
急性和慢性特应性皮炎（AD）自发性病变中组织嗜酸性粒细胞的频率和数量仍然存在争议，对皮肤中嗜酸性粒细胞的分布知之甚少。目的基于形态计量学数据定量描述急性和慢性阿尔茨海默病自发性病变组织嗜酸性粒细胞增多。方法对31例AD病变皮肤活检组织采用EG1、EG2、MBP、EPO和中性粒细胞弹性酶（NE）抗体免疫染色图像分析定量评估嗜酸性颗粒蛋白（EGP）沉积的方法。测定蛋白质沉积的频率、数量和分布，包括细胞外EGP沉积，作为嗜酸性粒细胞完全活化和脱颗粒的指标。同时进行嗜酸性粒细胞计数。对急性皮炎（海绵状病）和慢性皮炎（表皮增生）的组织病理学参数进行评分，以寻找与组织嗜酸性粒细胞增多的相关性。结果31例活检中有30例组织嗜酸性粒细胞增多。EG2蛋白含量最高，其次为EG1、MBP和EPO， NE含量极低。发现嗜酸性粒细胞浅表组织分布，伴
Background The frequency and amount of tissue eosinophilia in spontaneous lesions of acute and chronic atopic dermatitis (AD) are still a matter of controversy, and little is known about the distribution of eosinophilia in skin.Objectives To give a quantitative description of tissue eosinophilia in spontaneous lesions of acute and chronic AD based on morphometric data.Methods Thirty-one lesional skin biopsies of AD were evaluated using our recently described method for the quantitative assessment of eosinophilic granule protein (EGP) deposition by image analysis of immunostaining using the antibodies EG1, EG2, MBP, EPO and neutrophil elastase (NE). The frequency, amount and distribution of protein deposition including extracellular EGP deposition as an indicator of complete activation and degranulation of eosinophils were determined. Eosinophil count was performed in addition. Histopathological parameters of acute dermatitis (spongiosis) and chronic dermatitis (epidermal hyperplasia) were scored to look for a correlation with tissue eosinophilia.Results Tissue eosinophilia was found in nearly all biopsies (30 of 31). The most protein was detected by EG2, followed by EG1, MBP and EPO, with very small amounts of NE. A superficial tissue distribution of eosinophilia was found, with