Photocontrol of Tyrosine Phosphorylation in Mammalian Cells via Genetic Encoding of Photocaged Tyrosine
Photocontrol of Tyrosine Phosphorylation in Mammalian Cells via Genetic Encoding of Photocaged Tyrosine
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DOI：
10.1021/ja3046958
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发表时间：
2012-07-25
影响因子：
15
通讯作者：
Chin, Jason W.
中科院分区：
文献类型：
--
作者：
Arbely, Eyal;Torres-Kolbus, Jessica;Chin, Jason W.
我们报道了第一个位点特异性遗传编码的光笼酪氨酸成蛋白质在哺乳动物细胞。通过光笼化STAT1的Tyr701，我们证明了光控制哺乳动物细胞中酪氨酸磷酸化和信号转导是可能的。
We report the first site-specific genetic encoding of photocaged tyrosine into proteins in mammalian cells. By photocaging Tyr701 of STAT1 we demonstrate that it is possible to photocontrol tyrosine phosphorylation and signal transduction in mammalian cells.