Kaposi's sarcoma-associated herpesvirus gene expression in endothelial (Spindle) tumor cells
Kaposi's sarcoma-associated herpesvirus gene expression in endothelial (Spindle) tumor cells
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DOI：
10.1128/jvi.71.1.715-719.1997
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发表时间：
1997-01-01
影响因子：
5.4
通讯作者：
Hasse, AT
中科院分区：
文献类型：
--
作者：
Staskus, KA;Zhong, WD;Hasse, AT
最近在卡波西肉瘤（KS）中发现了一种新的人类疱疹病毒的DNA序列，这引发了这种病毒可能导致KS的猜测。然而，在像KS这样复杂的多细胞肿瘤中仅仅存在病毒，也可以被解释为乘客代理的证据。我们通过原位杂交研究KS相关疱疹病毒（KSHV）基因在KS肿瘤细胞中表达的特异性、恒定性和时间，寻求更有力的证据将其与肿瘤形成联系起来。在这里，我的文件表达的700个核苷酸的病毒RNA在每个KS肿瘤检查，从最早的组织学可识别的阶段，以先进的肿瘤，其中绝大多数可识别的梭形肿瘤细胞含有这种转录。除最早期病变外，在所有肿瘤细胞的较小部分中也检测到另外两种KSHV RNA。这些病毒RNA在该亚群中表达水平相对较低;因为这些RNA之一编码主要的病毒衣壳蛋白，这些细胞可能产生KSHV。我们没有发现这些KSHV基因在各种其他肿瘤和增殖过程中表达，但我们确实在前列腺组织中检测到病毒基因表达，支持KSHV性传播的可能机制。KS与KSHV基因表达之间的密切关系与KSHV直接参与KS病因和发病机制的假设一致。
The recent discovery of DNA sequences of a new human herpesvirus in Kaposi's sarcoma (KS) has fueled speculation that this virus might cause KS. The mere presence, however, of a virus in a complex multicellular tumor like KS could just as well be construed as evidence of a passenger agent. We sought stronger evidence linking the KS-associated herpesvirus (KSHV) to tumor formation by using in situ hybridization to investigate the specificity, constancy, and timing of KSHV gene expression in KS tumor cells. Here me document expression of a 700-nucleotide viral RNA in every KS tumor examined, from the earliest histologically recognizable stage to advanced tumors in which the vast majority of identifiable spindle tumor cells contain this transcript. Two other KSHV RNAs were also detected in a smaller fraction of the tumor cells in all but the earliest lesion. These viral RNAs,vere expressed to relatively low levels in this subset; because one of these RNAs encodes a major viral capsid protein, these cells mag be producing KSHV. We did not find these KSHV genes expressed in a variety of other tumors and proliferative processes, but we did detect viral gene expression in prostatic tissue, supporting a possible mechanism for sexual transmission of KSHV. The close relationship between KS and KSHV gene expression is consistent with the hypothesis that KSHV is directly involved in the etiology and pathogenesis of KS.