The relationship between susceptibility to retinoic acid treatment and protein kinase C alpha expression in murine melanoma cell lines.
The relationship between susceptibility to retinoic acid treatment and protein kinase C alpha expression in murine melanoma cell lines.
复制标题
鼠黑色素瘤细胞系对视黄酸治疗的敏感性与蛋白激酶 C α 表达之间的关系。
DOI：
10.1006/excr.1996.0054
复制
发表时间：
1996
影响因子：
3.7
通讯作者：
Combs,R
中科院分区：
文献类型：
--
作者：
Niles,RM;Combs,R
维甲酸（RA）诱导的B16小鼠黑色素瘤细胞分化伴随着PKCα蛋白量的大量增加。PKCα在这些细胞中的过表达导致更分化的表型。为了确定这些发现是否对小鼠黑色素瘤具有普遍适用性，我们研究了三种不同小鼠黑色素瘤细胞系对RA的敏感性与PKCα诱导之间的关系。RA抑制所有三种细胞系的贴壁依赖性生长，JB/MS是最敏感的，S91中间，和RPMI的影响最小。RA对JB/MS软琼脂集落形成也有抑制作用，但对RPMI影响不大。所有细胞系均表达PKCα，但不表达PKC β或PKC γ。RA诱导JB/MS中PKCα蛋白大量浓度依赖性增加（6- 10倍），S91中增加较小（2- 3倍），RPMI中PKCα诱导非常小。以前我们观察到PKCα的量随着培养的B16细胞密度的增加而增加。我们发现，这种密度依赖性增加PKCα发生在三个四黑色素瘤细胞系检查。提示PKCα在RA诱导的小鼠黑色素瘤细胞分化中起重要作用。
Retinoic acid (RA)-induced differentiation of B16 mouse melanoma cells is accompanied by a large increase in the amount of PKCα protein. Overexpression of PKCα in these cells results in a more differentiated phenotype. To determine if these findings had general applicability to murine melanomas, we investigated the relationship between sensitivity to RA and induction of PKCα in three different murine melanoma cell lines. RA inhibited the anchorage-dependent growth of all three cell lines, with JB/MS being the most sensitive, S91 intermediate, and RPMI the least affected. RA also inhibited soft agar colony formation in JB/MS, but had little effect on RPMI. All cell lines expressed PKCα, but not β or γ. RA induced a large concentration-dependent increase in PKCα protein in JB/MS (6- to 10-fold), a smaller increase in S91 (2- to 3-fold), and very little induction of PKCα in RPMI. Previously we had observed that the amount of PKCα increased with the density of B16 cells in culture. We found that this density-dependent increase in PKCα occurred in three out of four melanoma cell lines examined. These results suggest that PKCα plays an important role in RA-induced murine melanoma cell differentiation.