Identification and Expression Profiles of microRNA in Dolphin
Identification and Expression Profiles of microRNA in Dolphin
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DOI：
10.2108/zs150090
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发表时间：
2016-02
期刊：
影响因子：
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通讯作者：
Takao Segawa;Y. Kobayashi;S. Inamoto;Miwa Suzuki;Tomoko Endoh;T. Itou
中科院分区：
文献类型：
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作者：
Takao Segawa;Y. Kobayashi;S. Inamoto;Miwa Suzuki;Tomoko Endoh;T. Itou
近年来，microRNAs（miRNAs）作为生物标志物的作用受到关注，因为它们在血清和血浆中稳定存在，并且在特定器官中表达，随着器官损伤而增加。因此，miRNAs可能是非常有用的生物标志物，用于监测海豚的健康和状况，并用于检测水族馆中的疾病。在这里，从海豚的肺、肝和脾中制备了一个小RNA文库，然后确定了15种不同组织的miRNA表达模式。我们在海豚小RNA文库中鉴定了62个保守的miRNA同源物，并在特定组织中发现了高表达的miRNA：miR-125 b和miR-221在脑中高表达，miR-23 b在心脏中高表达，miR-199 a和miR-223在肺中高表达，miR-122- 5 p在肝脏中高表达。这些组织富集的miRNAs中的一些可能是有用的，作为海豚器官损伤的特异性和敏感的诊断血液生物标志物。
Recently, microRNAs (miRNAs) are focused on the role of biomarker because they are stable in serum and plasma, and some of them express in the specific organs and increase with the organ injury. Thus miRNAs may be very useful as biomarkers for monitoring the health and condition of dolphins and for detecting disorders in aquariums. Here, a small RNA library was made from dolphin lung, liver and spleen, and miRNA expression patterns were then determined for 15 different tissues. We identified 62 conserved miRNA homologs in the dolphin small RNA library and found high expression miRNAs in specific tissues: miR-125b and miR-221 were highly expressed in brain, miR-23b in heart, miR-199a and miR-223 in lung, and miR-122-5p in liver. Some of these tissue-enriched miRNAs may be useful as specific and sensitive diagnostic blood biomarkers for organ injury in dolphins.