Guidelines for Micro-Computed Tomography Analysis of Rodent Dentoalveolar Tissues.
Guidelines for Micro-Computed Tomography Analysis of Rodent Dentoalveolar Tissues.
复制标题
啮齿动物牙槽组织的微型计算机断层扫描分析指南。
作者：
Chavez MB;Chu EY;Kram V;de Castro LF;Somerman MJ;Foster BL
微计算机断层扫描（μCT）已成为分析矿化和非矿化组织的关键，因此在生命科学中具有广泛的应用。然而，由于缺乏标准化的方法和协议来扫描，分析和报告数据，通常很难准确理解如何进行分析，如何解释结果，以及研究结果是否可以与其他模型和研究进行广泛比较。在分析牙槽骨复合体时，由于存在四种不同的矿化组织：牙釉质、牙本质、牙骨质和牙槽骨，这个问题变得更加复杂。此外，这些硬组织与邻近的软组织、牙髓和牙周韧带（PDL）接触，形成复杂的器官。本文在总结前人和我们自己利用μCT分析啮齿动物牙槽骨组织的经验的基础上，介绍了利用μCT成功分析具有相似或不同成分、密度和形态特征的牙槽骨组织的技术。我们的目标是为啮齿动物牙槽骨组织的μCT分析提供实用指南，包括优化扫描参数（滤波器，电压，体素大小和积分时间），可重复定向样本，定义感兴趣区域和体积，分割和细分组织，解释结果以及报告方法和结果的方法。我们包括在遗传工程小鼠模型上进行的分析的说明性例子，在牙釉质，牙本质，牙骨质和牙槽骨的表型。这些建议旨在提高透明度和可重复性，促进最佳实践，并提供一个基本框架，将μCT分析应用于牙槽骨复合体，也可以外推到身体的各种其他组织。版权所有© 2021作者。JBMR Plus由Wiley Periodicals LLC出版。美国骨与矿物质研究协会（American Society for Bone and Mineral Research）微型计算机断层扫描（microCT）已成为分析矿化组织的关键。缺乏标准化的方法和协议的扫描，分析和报告的数据，使它难以理解如何进行分析和报告，一个问题复杂的牙槽骨复合体的存在下，四个不同的矿化组织：釉质，牙本质，牙骨质，和牙槽骨。我们的目标是为microCT分析提供实用指南，包括如何优化扫描参数（滤波器，电压，体素大小和积分时间），可重复地定向样本，定义感兴趣的区域和体积，分割和细分组织，解释发现，并报告方法和结果。
Micro–computed tomography (μCT) has become essential for analysis of mineralized as well as nonmineralized tissues and is therefore widely applicable in the life sciences. However, lack of standardized approaches and protocols for scanning, analyzing, and reporting data often makes it difficult to understand exactly how analyses were performed, how to interpret results, and if findings can be broadly compared with other models and studies. This problem is compounded in analysis of the dentoalveolar complex by the presence of four distinct mineralized tissues: enamel, dentin, cementum, and alveolar bone. Furthermore, these hard tissues interface with adjacent soft tissues, the dental pulp and periodontal ligament (PDL), making for a complex organ. Drawing on others' and our own experience analyzing rodent dentoalveolar tissues by μCT, we introduce techniques to successfully analyze dentoalveolar tissues with similar or disparate compositions, densities, and morphological characteristics. Our goal is to provide practical guidelines for μCT analysis of rodent dentoalveolar tissues, including approaches to optimize scan parameters (filters, voltage, voxel size, and integration time), reproducibly orient samples, define regions and volumes of interest, segment and subdivide tissues, interpret findings, and report methods and results. We include illustrative examples of analyses performed on genetically engineered mouse models with phenotypes in enamel, dentin, cementum, and alveolar bone. The recommendations are designed to increase transparency and reproducibility, promote best practices, and provide a basic framework to apply μCT analysis to the dentoalveolar complex that can also be extrapolated to a variety of other tissues of the body. © 2021 The Authors. JBMR Plus published by Wiley Periodicals LLC. on behalf of American Society for Bone and Mineral Research. Micro‐computed tomography (microCT) has become essential for analysis of mineralized tissues. Lack of standardized approaches and protocols for scanning, analyzing, and reporting data makes it difficult to understand how analyses were performed and reported, a problem compounded in the dentoalveolar complex by the presence of four distinct mineralized tissues: enamel, dentin, cementum, and alveolar bone. We aim to provide practical guidelines for microCT analysis, including how to optimize scan parameters (filters, voltage, voxel size, and integration time), reproducibly orient samples, define regions and volumes of interest, segment and subdivide tissues, interpret findings, and report methods and results.