Identification of a short form of the P2xR1-purinoceptor subunit produced by alternative splicing in the pituitary and cochlea.
Identification of a short form of the P2xR1-purinoceptor subunit produced by alternative splicing in the pituitary and cochlea.
复制标题
鉴定垂体和耳蜗中通过选择性剪接产生的 P2xR1-嘌呤受体亚基的短形式。
DOI：
10.1006/bbrc.1995.1998
复制
发表时间：
1995
影响因子：
3.1
通讯作者：
Ryan,AF
中科院分区：
文献类型：
--
作者：
Housley,GD;Greenwood,D;Bennett,T;Ryan,AF
一个截短形式的P2 xR 1嘌呤受体亚基（我们指定P2 xR 1 -2）检测到在大鼠垂体和分泌上皮组织（纹血管）的耳蜗使用RT-PCR的固相cDNA文库。从输精管、脑和显微解剖的耳蜗感觉上皮组织（包括Corti器、球囊和壶腹嵴）获得对应于P2 xR 1嘌呤受体亚基（1）的PCR产物。克隆和测序结果表明，P2 xR 1 -2产品包括一个85 bp的插入区域对应于一个新的C-末端的第二跨膜结构域和继续作为胞质结构域。插入序列的前51 bp后的终止密码子序列有效地截短了该亚基，与先前发表的P2 xR 1（−1）序列相比，将最终的胞质结构域减少了90%，从而将整个肽减少了约25%。在截短形式中丢失的受体区域编码许多富含丝氨酸/脯氨酸的区域，这些区域可能充当潜在的细胞内调节位点。
A truncated form of the P2xR1 purinoceptor subunit (which we designate P2xR1-2) was detected in rat pituitary gland and the secretory epithelial tissue (stria vascularis) of the cochlea using RT-PCR of solid-phase cDNA libraries. PCR products corresponding to the P2xR1 purinoceptor subunit (1) were obtained from vas deferens, brain and microdissected cochlear sensory epithelial tissues including organ of Corti, sacculus and crista ampullaris. Cloning and sequencing revealed that the P2xR1-2 product included an 85 - bp insertion in a region correponding to a novel C-terminal end of the second membrane spanning domain and continuing as the cytoplasmic domain. A stop condon sequence after the first 51 bp of the insert effectively truncates this subunit, reducing the final cytoplasmic domain by 90% compared with the previously published P2xR1(−1) sequence, thereby reducing the overall peptide by approximately 25%. The region of the receptor lost in the truncated version coded for a number of serine/proline rich regions which may act as potential intracellular regulatory sites.