Swimming performance and energy metabolism of rainbow trout, common carp and gibel carp respond differently to sublethal copper exposure
Swimming performance and energy metabolism of rainbow trout, common carp and gibel carp respond differently to sublethal copper exposure
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DOI：
10.1016/j.aquatox.2006.07.017
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发表时间：
2006-10-25
影响因子：
4.5
通讯作者：
Blust, R.
中科院分区：
文献类型：
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作者：
De Boeck, G.;van der Ven, K.;Blust, R.
我们比较了亚致死水中铜暴露对虹鳟鱼（Oncorhynchus mykiss）和普通鲤鱼（Cyprinus carpio）、异育银鲫（Carassius auratus gibelio）等不太敏感的鲤类的游泳性能和呼吸速率的影响。这些鲤类对铜中毒的抵抗力更强，在游泳表现和呼吸行为上与鳟鱼不同。测定1 μ M Cu亚致死暴露28天的临界游泳速度（U-crit）、耗氧量、血浆氨和肌氨、乳酸和pH值。在前12 ~ 24 h内，虹鳟、普通鳟鱼和gibel的U-crit分别下降了48%、31%和13%。吉伯鱼恢复得很快，游泳性能没有进一步下降。虹鳟鱼和鲤鱼的游泳能力仅部分恢复。这三个物种在最初的几个小时到几天内都表现出相似的血浆氨峰值，随着时间的推移，肌肉氨积累更为缓慢。虹鳟鱼没有出现呼吸压力的迹象，而普通鲤鱼在暴露24小时后经历了短暂的氧气消耗减少和无氧代谢。同时，异育银鲫的耗氧量也有所降低，但没有发现无氧代谢的迹象。铜在三种鱼的鳃中迅速积累到相似的水平（第3天干重36-39 μ g g(-1)），之后积累趋于平稳。虹鳟鱼肝脏组织从一开始就具有较高的Cu水平，且Cu浓度没有额外的积累。相比之下，普通鲤鱼肝脏从第3天开始就有显著的铜积累，而异育银鲫肝脏的铜积累要慢得多，从第7天开始就很显著。有趣的是，铜在血浆和肾脏中的积累模式揭示了肾脏在异育银鲫铜稳态中的重要作用。(c) 2006 Elsevier B.V.版权所有
We compared the effects of sublethal waterborne copper exposure on swimming performance and respiration rates in rainbow trout, Oncorhynchus mykiss, with those in less sensitive cyprinid species such as common carp, Cyprinus carpio, and gibel carp, Carassius auratus gibelio. These cyprinids are considerably more resistant to Cu intoxication, and differ from trout in swimming performance and respiratory behaviour. Critical swimming speed (U-crit), oxygen consumption, plasma ammonia and muscle ammonia, lactate and pH were measured during a 28-day sublethal exposure to 1 mu M Cu. U-crit decreased with 48, 31 and 13% within the first 12-24 h for rainbow trout, common and gibel respectively. Gibel carp recovered quickly and experienced no further reduction in swimming performance. Recovery of swimming capacity in rainbow trout and common carp was only partial. All three species displayed similar plasma ammonia peaks in the first hours to days, and a more gradual muscle ammonia accumulation over time. Whereas no signs of respiratory stress occurred in rainbow trout, common carp experienced a transient reduction in oxygen consumption combined with anaerobic metabolism after 24 h of exposure. At the same time, oxygen consumption was also reduced in gibel carp, but no signs of anaerobic metabolism were detected. Cu accumulated quickly to similar levels (36-39 mu g g(-1) dry weight at day 3) in the gills of all three species, after which accumulation leveled off. Liver tissue of rainbow trout had a high Cu level from the start, and Cu concentration did not show any additional accumulation. In contrast, common carp liver showed a significant Cu accumulation from day 3 onwards, while accumulation in gibel livers was much slower and was significant from day 7 onwards. Interestingly, Cu accumulation patterns in plasma and kidney revealed a possibly important role for the kidney in Cu homeostasis of gibel carp. (c) 2006 Elsevier B.V. All rights reserved.