DIFFERENCE SPECTRA AND EXTINCTION COEFFICIENTS OF P700
DIFFERENCE SPECTRA AND EXTINCTION COEFFICIENTS OF P700
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DOI：
10.1016/0005-2728(72)90147-8
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发表时间：
1972-01-01
期刊：
影响因子：
--
通讯作者：
KE, B
中科院分区：
文献类型：
--
作者：
HIYAMA, T;KE, B
根据 N,N,N',N'-四甲基对苯二胺与 P700 偶联的氧化还原反应引起的闪光引起的吸收变化，经洋地黄皂苷处理的菠菜光系统-I 颗粒的消光系数差异为 64 mequiv−1·cm−1 (700 nm)，经 Triton 处理的鱼腥草光系统 I 颗粒的差异消光系数为 70 mequiv−1·cm−1 (701 nm)变量。经过适当校正后，该值与使用细胞色素眼虫细胞色素 552 作为直接电子供体的方法获得的值一致。这些消光系数和从指数恢复阶段构建的差异光谱进一步强化了 P700 是叶绿素的一种形式的概念。
From the flash-induced absorption changes due to the redox reaction ofN,N,N′,N′-tetramethyl-p-phenylenediamine coupled with P700, the difference extinction coefficients were determined to be 64 mequiv−1·cm−1at700 nm for digitonin-treated Photosystem-I particles of spinach, and 70 mequiv−1·cm−1at701 nm for Triton-treated Photosystem-I particles ofAnabaena variabilis. The values were consistent with those obtained by the method using either cytochromecorEuglenacytochrome 552 as direct electron donor after proper corrections. These extinction co-efficients and the difference spectra constructed from the exponential recovery phases further strengthened the notion that P700was a form of chlorophylla.