Investigation of novel autoantibodies in Sjogren's syndrome utilizing Sera from the Sjogren's international collaborative clinical alliance cohort.
Investigation of novel autoantibodies in Sjogren's syndrome utilizing Sera from the Sjogren's international collaborative clinical alliance cohort.
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DOI：
10.1186/s12886-015-0023-1
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发表时间：
2015-04-10
影响因子：
2
通讯作者：
Ambrus JL Jr
中科院分区：
文献类型：
--
作者：
Suresh L;Malyavantham K;Shen L;Ambrus JL Jr
干燥综合征（SS）是一种主要影响唾液腺和泪腺的慢性自身免疫性疾病。目前的SS诊断标准以抗ro和抗la作为血清学指标。SS动物模型已鉴定出新的自身抗体，抗唾液腺蛋白1 （SP1）、抗碳酸酐酶6 （CA6）和腮腺分泌蛋白（PSP）。与抗ro和抗la相比，这些新型抗体出现在SS早期的动物体内。目前的研究旨在评估来自干燥国际合作临床联盟（SICCA）的干眼、口干和唇干活检患者血清中的这些新型自身抗体，以确定它们是否确实识别SS的严重程度低于表达抗ro和抗la的患者。从SICCA登记处获得唇活检中淋巴细胞病灶每4mm2为0 (F = 0), <1 （F <1）或bbbb3 （F bbbb3）的患者的血清。采用ELISA法检测血清的抗ro、抗la、抗sp1、抗ca6和抗psp水平。在眼干口干但F = 0的患者中，与F <1组（p = 0.032）或F < 3组（p = 0.006）相比，anti- CA6表达增加。F0组中anti-PSP和anti-SP1的表达量虽有增高的趋势，但由于F0组中数量较少，因此anti-PSP和anti-SP1的表达量均无统计学意义。另一方面，与F <1 （p = .0021）和F >3 （p = .0003）组相比，F0组anti-Ro的表达明显降低。F0组抗la表达较F <1和f>.3组降低，但差异无统计学意义。抗- ro和抗- la比抗- sp1、抗- ca6和抗- psp更能识别SS患者。需要更多的研究来确定这些不同的自身抗体在SS过程中的表达时间和/或它们是否在不同临床表现的患者中表达。
Sjogren’s syndrome (SS) is a chronic autoimmune disease mainly affecting salivary and lacrimal glands. Current diagnostic criteria for SS utilize anti-Ro and anti-La as serological markers. Animal models for SS have identified novel autoantibodies, anti-salivary gland protein 1 (SP1), anti-carbonic anhydrase 6 (CA6) and parotid secretory protein (PSP). These novel antibodies are seen in the animals at an earlier stage of SS than anti-Ro and anti-La. The current studies were designed to evaluate these novel autoantibodies in the sera of well-characterized patients with dry eyes and dry mouth and lip biopsies from the Sjogren’s International Collaborative Clinical Alliance (SICCA) to determine if they indeed identify SS with less severe disease than patients expressing anti-Ro and anti-La. Sera were obtained from SICCA registry in patients for whom lymphocytic foci per 4 mm2 on the lip biopsies was either 0 (F = 0), <1 (F <1) or > 3 (F >3). ELISA assays were utilized to evaluate these sera for anti-Ro, anti-La, anti-SP1, anti-CA6, and anti-PSP. In patients with dry eyes and dry mouth but F = 0, increased expression of anti- CA6 was noted compared to the F <1 group (p = .032) or the F > 3 group (p = .006). Neither anti-PSP nor anti-SP1 reached statistical significance because of the small numbers in the F0 group, although there was a trend for their expression to be higher in the F0 group. On the other hand, the expression of anti-Ro was significantly reduced in the F0 group compared to the F <1 (p = .0021) and F > 3 (p = .0003) groups. The reduced expression of anti-La in the F0 group compared to the F <1 and F > 3 groups did not quite reach statistical significance. Anti-Ro and anti-La identify patients with SS and more severe disease than anti-SP1, anti-CA6, and anti-PSP. More studies are needed to identify the timing in the course of SS when these different autoantibodies are expressed and/or whether they are expressed in patients with different clinical manifestations.
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影响因子：
27.4
作者：
Rasmussen A;Ice JA;Li H;Grundahl K;Kelly JA;Radfar L;Stone DU;Hefner KS;Anaya JM;Rohrer M;Gopalakrishnan R;Houston GD;Lewis DM;Chodosh J;Harley JB;Hughes P;Maier-Moore JS;Montgomery CG;Rhodus NL;Farris AD;Segal BM;Jonsson R;Lessard CJ;Scofield RH;Sivils KL
通讯作者：
Sivils KL
影响因子：
4.9
作者：
Low HZ;Witte T
通讯作者：
Witte T
影响因子：
8.6
作者：
Shen, Long;Suresh, Lakshmanan;Ambrus, Julian L.
通讯作者：
Ambrus, Julian L.
影响因子：
158.5
作者：
Arbuckle, MR;McClain, MT;Harley, JB
通讯作者：
Harley, JB
影响因子：
--
作者：
Daniels, Troy E.;Criswell, Lindsey A.;Shiboski, Caroline;Shiboski, Stephen;Lanfranchi, Hector;Dong, Yi;Schiodt, Morten;Umehara, Hisanori;Sugai, Susumu;Challacombe, Stephen;Greenspan, John S.
通讯作者：
Greenspan, John S.